CAJ | 학술논문

目的探讨选择性消化道脱污染对烫伤大鼠组织白细胞介素-18 mRNA表达的影响及脏器保护作用。方法采用大鼠30％体表面积Ⅲ度烫伤延迟复苏模型，肠、肺组织IL-18、IFN-γ mRNA含量采用逆转录聚合酶链式反应检测；ELISA法测定IFN-γ含量；同时检测肠、肺功能指标。结果肠、肺组织IL-18、IFN-γ mRNA表达较伤前有显著升高，烫伤后8 h达高峰，且一直持续至伤后24 h，给予SDD预治疗后可不同程度抑制IL-18、IFN-γ mRNA的表达；严重烫伤后，出现广泛的脏器功能损害，预防性SDD处理可不同程度地保护脏器。结论肠、肺组织IL-18、IFN-γ mRNA表达在烫伤后显著增多，并逐渐升高，后者对脏器具有重要影响。SDD预治疗后可不同程度抑制IL-18 mRNA的表达并保护脏器。
목적탐토선택성소화도탈오염대탕상대서조직백세포개소-18 mRNA표체적영향급장기보호작용。방법채용대서30％체표면적Ⅲ도탕상연지복소모형，장、폐조직IL-18、IFN-γ mRNA함량채용역전록취합매련식반응검측；ELISA법측정IFN-γ함량；동시검측장、폐공능지표。결과장、폐조직IL-18、IFN-γ mRNA표체교상전유현저승고，탕상후8 h체고봉，차일직지속지상후24 h，급여SDD예치료후가불동정도억제IL-18、IFN-γ mRNA적표체；엄중탕상후，출현엄범적장기공능손해，예방성SDD처리가불동정도지보호장기。결론장、폐조직IL-18、IFN-γ mRNA표체재탕상후현저증다，병축점승고，후자대장기구유중요영향。SDD예치료후가불동정도억제IL-18 mRNA적표체병보호장기。
Objective　To investigate the effect of selective decontamination of the digestive tract on interleukin-18 mRNA expression and organ dysfunction in thermal injured rats.Methods　54 male Wistar were randomly divided into three groups: normal controls，30％ Ⅲ injury group，and selective decontamination of the digestive tract (SDD) treatment group.The expression of cytokine mRNAs was semi-quantitated by the reverse transcription polymerase chain reaction (RT-PCR) using GAPDH as an internal standard. Enzyme-linked immunosorbent assay (ELISA) was performed for IFN-γ measurement.Results　IL-18、IFN-γ mRNA expression significantly increased in intestine,lung at 2 hours，peaking at 8 hours，and a high level was maintained up to 24 hours.However,treatment with SDD could markedly inhibit cytokine mRNAs expression and protect organ function.Conclusion　This study suggests that extensive burns can lead to a marked elevation of tissue IL-18 and IFN-γ mRNA expression in vital organs，which might be associated with the damage of organ function.Treatment with SDD can effectively block the IL-18 pathway and the excessive inflammation response.