CAJ | 학술논문

目的:研究药物敏感虫株伯氏疟原虫K173株及其在本芴醇压力下产生的本芴醇抗性株之间基因表达的差异状况,克隆抗性相关基因.方法:采用mRNA差异显示技术寻找相关基因,并用Northern杂交证实.结果:采用24种引物组合进行mRNA差异显示,克隆到30余个差异表达基因片段,对其中8个进行了Northern杂交鉴定及测序.结果表明,与敏感株相比,克隆CB52在本芴醇抗性株中表达明显增多,而在氯喹抗性株中的表达有所减少,说明克隆CB52确与本芴醇抗性相关,序列同源性检索表明CB52序列与恶性疟原虫环亲蛋白基因有微弱同源性.向GenBank登录8条新基因序列.结论:克隆CB52基因表达水平的改变与伯氏疟原虫本芴醇抗性的产生相关,疟原虫对本芴醇的抗性机制在基因水平不同于氯喹抗性.
목적:연구약물민감충주백씨학원충K173주급기재본물순압력하산생적본물순항성주지간기인표체적차이상황,극륭항성상관기인.방법:채용mRNA차이현시기술심조상관기인,병용Northern잡교증실.결과:채용24충인물조합진행mRNA차이현시,극륭도30여개차이표체기인편단,대기중8개진행료Northern잡교감정급측서.결과표명,여민감주상비,극륭CB52재본물순항성주중표체명현증다,이재록규항성주중적표체유소감소,설명극륭CB52학여본물순항성상관,서렬동원성검색표명CB52서렬여악성학원충배친단백기인유미약동원성.향GenBank등록8조신기인서렬.결론:극륭CB52기인표체수평적개변여백씨학원충본물순항성적산생상관,학원충대본물순적항성궤제재기인수평불동우록규항성.
Objective:To isolate genes related to benflumetol resistance in the rodent malaria P.berghei K173 strain.Methods:The mRNA differential display technique was used to identify differentially expressed genes between drug-sensitive P.berghei K173 strain and benflumetol-resistant strain selected under benflumetol pressure.Northern blot was performed in order to determine the genes actually derived from maternal strains.Results:PCR reactions were prepared respectively with twenty-four pairs of primers in each of the strains.Thirty-three differentially displayed genes fragments were cloned and eight of them were sequenced. The eight sequences were novel ESTs and were submitted to GenBank. Homology searching revealed that clone CB52 was slightly homologous to P.falciparum cyclophilin gene. Hybridization confirmed that the gene fragment CB52 was upregulated in the benflumetol-resistant parasite strain, while downregulated in the chloroquine-resistant strain.Conclusions:CB52 fragment was involved in the benflumetol resistance in P.berghei and the genetic mechanisms of drug-resistance to benflumetol and chloroquine were different.