CAJ | 학술논문

探讨人糖基化磷脂酰肌醇特异性磷脂酶D (GPI-PLD)的 cDNA及其基因组基因的结构。从人骨髓基质细胞克隆出的GPI-PLD cDNA中发现，它能编码信号肽，其编码的酶蛋白成熟肽为817个氨基酸残基。该GPI-PLD cDNA与从人肝组织和胰腺组织克隆到的GPI-PLD cDNA的碱基序列的同源性分别为95%和99%。人GPI-PLD基因组基因定位于6p22.1～22.3区域，包含25个外显子，其上游调控区具有启动子（TATA 盒与CAAT盒）、增强子核心序列（TGGAAG）及同源转换盒Pit-1/GHF-1结合序列（TATGCATAA）等顺式作用元件。
탐토인당기화린지선기순특이성린지매D (GPI-PLD)적 cDNA급기기인조기인적결구。종인골수기질세포극륭출적GPI-PLD cDNA중발현，타능편마신호태，기편마적매단백성숙태위817개안기산잔기。해GPI-PLD cDNA여종인간조직화이선조직극륭도적GPI-PLD cDNA적감기서렬적동원성분별위95%화99%。인GPI-PLD기인조기인정위우6p22.1～22.3구역，포함25개외현자，기상유조공구구유계동자（TATA 합여CAAT합）、증강자핵심서렬（TGGAAG）급동원전환합Pit-1/GHF-1결합서렬（TATGCATAA）등순식작용원건。
To explore the cDNA and its genomic gene structure of human glycosylphosphatidylinositol specific phospholipase D (GPI-PLD), the stromal cells from human bone marrow were cultured, and the GPI-PLD cDNA was successfully cloned from stromal cells (GenBank Accession AY007546). After analyzing this cDNA, we found that it codes the signal peptide as well as the mature peptide (817 AA) of GPI-PLD. Compared with the cDNA cloned from human pancreas and liver, the homology of the cDNA is 99% and 95% respectively. After searching GenBank, we aligned the genomic gene of the GPI-PLD by DNATools software, and found that the GPI-PLD genomic gene was located in the human 6p22.1-22.3 and contained 25 exons, TATA box, CAAT box, enhancer and the sequence binding homo domain of Pit-1/GHF-1.