CAJ | 학술논문

为了监测安徽省现流行的麻疹野毒株，在1998、1999年的2起麻疹暴发中，用绒猴淋巴母细胞（B95a）分离到4株麻疹野病毒，通过血凝试验、血吸附试验、中和试验对分离到的麻疹病毒进行特性鉴定。该4株病毒无血凝和血球吸附活性，我国人类疫苗免疫后，血清能中和这4株病毒，但中和此野毒株的滴度低于中和疫苗株滴度的2倍。逆转录聚合酶链反应（RT－PCR）和其产物的基因序列分析提示，该4株病毒属于麻疹病毒第八基因组（H group）。继续监测我国各省麻疹流行株的基因和抗原变异，对我国强化麻疹控制及麻疹消除、消灭十分必要
위료감측안휘성현류행적마진야독주，재1998、1999년적2기마진폭발중，용융후림파모세포（B95a）분리도4주마진야병독，통과혈응시험、혈흡부시험、중화시험대분리도적마진병독진행특성감정。해4주병독무혈응화혈구흡부활성，아국인류역묘면역후，혈청능중화저4주병독，단중화차야독주적적도저우중화역묘주적도적2배。역전록취합매련반응（RT－PCR）화기산물적기인서렬분석제시，해4주병독속우마진병독제팔기인조（H group）。계속감측아국각성마진류행주적기인화항원변이，대아국강화마진공제급마진소제、소멸십분필요
This study was to survey the current wild-type measles virus that circulated in Anhui Province. 4 wild-type measles viruses were isolated in B95a cells from 2 outbreaks during 1998 and 1999. The virus characteristics were identified by hemagglutination test, haemadsorption test and neutralization test. The results showed that the 4 wild-type measles viruses did not have hemagglutination and haemadsorption activities and the sera against Chinese vaccine (S191) could neutralize all of the 4 wild-type measles virus strains, but the neutralizing titer was two times lower compared with that of S191. RT-PCR and its product sequence analysis indicated that the 4 wild-type measles virus strains belong to H genetic group. In order to control and eliminate measles, it is important to survey continually the genetic and antigenic shifts of the prevalent measles viruses circulating in China.