西南农业学报
西南農業學報
서남농업학보
SOUTHWEST CHINA JOURNAL OF AGRICULTURAL SCIENCES
2001年
1期
96-98
,共3页
熊鹰%童云霞%唐利民%姜邻
熊鷹%童雲霞%唐利民%薑鄰
웅응%동운하%당이민%강린
菌种%琼脂斜面%自然基质%液体石蜡%保藏方法
菌種%瓊脂斜麵%自然基質%液體石蠟%保藏方法
균충%경지사면%자연기질%액체석사%보장방법
通过对三种封口方式保藏管的基质形态和菌丝回接成活率比较,证实有孔胶塞是一种较理想的封口方式,它能协调保水与通气的矛盾。对琼脂斜面低温保藏、自然基质柱状低温保藏和液体石蜡常温保藏比较表明,前两者结合使用,是一种简单易行的短、长期兼顾的保种方法。
通過對三種封口方式保藏管的基質形態和菌絲迴接成活率比較,證實有孔膠塞是一種較理想的封口方式,它能協調保水與通氣的矛盾。對瓊脂斜麵低溫保藏、自然基質柱狀低溫保藏和液體石蠟常溫保藏比較錶明,前兩者結閤使用,是一種簡單易行的短、長期兼顧的保種方法。
통과대삼충봉구방식보장관적기질형태화균사회접성활솔비교,증실유공효새시일충교이상적봉구방식,타능협조보수여통기적모순。대경지사면저온보장、자연기질주상저온보장화액체석사상온보장비교표명,전량자결합사용,시일충간단역행적단、장기겸고적보충방법。
The configuration of culture media and surviving rate of myceliumreturning inoculation were compared by three sealing up test tube m anners of conserving tube.The result showed that gum stopper with hole was a rel atively perfect sealing up way.It could coordinate the contravention between kee ping water and aerating.This paper also studied the conserving tube peculiaritie s in agar-slant low temperature,nature culture media pole shape low temperature and liquid olefin normal temperature,respectively.The result indicated that for mer both combined to use was a simple and plausible method to conserve strains i n long and short term.