CAJ | 학술논문

将外源λDNA导入受体普通小麦品系814527，获得一雄性不育变异体。对该不育变异体和受体花粉母细胞减数分裂过程进行观察。结果发现，不育变异体花粉母细胞染色体异常比率达到18%，而受体花粉母细胞染色体异常比例为0.8%，二者存在明显差异。染色体异常类型主要有单价体、染色体落后、染色体断片、染色体桥、微核及二分体、四分体异常等。染色体异常可能是外源DNA导入受体后，整合进受体染色体中的DNA片段影响其正常的遗传过程而造成的。花粉母细胞减数分裂过程染色体异常可以引起部分花粉败育，但不是该变异体败育的主要原因。
장외원λDNA도입수체보통소맥품계814527，획득일웅성불육 변이체。대해불육변이체화수체화분모세포감수분렬과정진행관찰。결과발현，불육변이체 화분모세포염색체이상비솔체도18%，이수체화분모세포염색체이상비례위0.8%，이자존재 명현차이。염색체이상류형주요유단개체、염색체락후、염색체단편、염색체교、미핵급이 분체、사분체이상등。염색체이상가능시외원DNA도입수체후，정합진수체염색체중적DNA편 단영향기정상적유전과정이조성적。화분모세포감수분렬과정염색체이상가이인기부분화분 패육，단불시해변이체패육적주요원인。
A male sterile mutant was obtained after injection of e xogenous λ DNA into wheat line 814527. Meiosis of pollen mother cells(PMC) of t he mutant and its receptor(line 814527) were observed. The results showed that t h e frequency of chromosomal variation of the sterile line was 18%,and that of the receptor was 0.8%. The main types of variation included univalent, chromosome lagging, chromosome fragment, chromosome bridge, micronucleus, abnormal ditrad a nd tetrad. The fragment of DNA injected into the receptor may influence the norm al genetic process of chromosomes in pollen mother cells, and this may cause var iations of chromosomes. The chromosome variation in meiosis may cause a part of pollen mother cells to abort, but it is not the main cause of abortion.