海洋科学
海洋科學
해양과학
MARINE SCIENCES
2000年
11期
35-38
,共4页
三角褐指藻%半连续培养%一次性培养%更新率%均匀设计
三角褐指藻%半連續培養%一次性培養%更新率%均勻設計
삼각갈지조%반련속배양%일차성배양%경신솔%균균설계
Phaeodactylum tricornutum%Semicontinuous culture%Batch culture%Renewal rate%Uniform design
采用均匀设计，在室温下研究了半连续培养中氮、磷浓度，更新率对三角褐指藻采收量的动态变化关系。结果显示，对16 d更新期，氮、磷浓度分别在0．5～35．9mg／L和0．3～12．1mg／L范围对采收量无影响，但氮浓度在8 d前宜用0．5mg／L。更新率增加，藻浓度下降，藻生长率增大。据16 d方程，最适更新率为18．2％，每ml培养体积日采收量可达667 000细胞。
採用均勻設計，在室溫下研究瞭半連續培養中氮、燐濃度，更新率對三角褐指藻採收量的動態變化關繫。結果顯示，對16 d更新期，氮、燐濃度分彆在0．5～35．9mg／L和0．3～12．1mg／L範圍對採收量無影響，但氮濃度在8 d前宜用0．5mg／L。更新率增加，藻濃度下降，藻生長率增大。據16 d方程，最適更新率為18．2％，每ml培養體積日採收量可達667 000細胞。
채용균균설계，재실온하연구료반련속배양중담、린농도，경신솔대삼각갈지조채수량적동태변화관계。결과현시，대16 d경신기，담、린농도분별재0．5～35．9mg／L화0．3～12．1mg／L범위대채수량무영향，단담농도재8 d전의용0．5mg／L。경신솔증가，조농도하강，조생장솔증대。거16 d방정，최괄경신솔위18．2％，매ml배양체적일채수량가체667 000세포。
This experiment was conducted to determine the dynamic effects of nitrogen, phosphorus concentration and renewal rate on recovery yields of Phaeodactylum tricornutum in semicontinuous cultures at room temperature by uniform design. The results demonstrated that nitrogen concentration ranging from 0.5 rg / L to 35.9 rg/L and plosphorus concentration ranging from 0.3 mg/L to 12.1 mg/L had no influences upon recovery yields during 16 d dilution, but 0.5 rg/L of nitrogen concentration was suggested before 8 d. Cell density decreased and growth rate increased while increasing renewal rate. The optimal renewal rate of 18.2% was postulated according to 16 d equation, and recovery yields of 66.7×104 cells per ml volume
of culture per day were obtained with this renewal rate.
