CAJ | 학술논문

于1998年3月-1999年2月，在山东农业大学采集泰山赤鳞鱼，采用精子载体法进行MT-hGH基因导入的研究。将泰山赤鳞鱼的精子在保存液内与含有MT-hGH基因的重组质粒保温30min，然后用于人工授精，共孵化出363尾幼鱼。待鱼苗长至5cm左右时，对其中的39尾进行PCR和Southern杂交检测。结果表明，有7尾鱼苗带有MT- hGH基因，转基因鱼的阳性率为17.95%。
우1998년3월-1999년2월，재산동농업대학채집 태산적린어，채용정자재체법진행MT-hGH기인도입적연구。장태산적린어적정자재보존액내 여함유MT-hGH기인적중조질립보온30min，연후용우인공수정，공부화출363미유어。대어묘 장지5cm좌우시，대기중적39미진행PCR화Southern잡교검측。결과표명，유7미어묘대유MT- hGH기인，전기인어적양성솔위17.95%。
Transfering MT-hGH gene into Varicorhinus tr acrolepis with sperms as vectors was carried out from March, 1998 to February, 1999. The Varicorhinus tracrolepis sperms were incubated with MT-hGH gene i n dilute citrate solution for 30 minutes. Then they were used in artificial inse mination. 997 eggs were inseminated with the treated sperms and 755 of them were fertilized. 363 fish were hatched in 3-4 days. When the fish grew to about 5cm , genomic DNA was isolated from 39 fish. The samples were tested by PCR and Sout hern blot hybridization. The result shows that 7 fish contained MT-hGH gene. The positive rate was about 17.95%.