CAJ | 학술논문

作者对来自牡丹江市林业医院和海林林场的34份尿液标本进行了5s-23srRNA基因间隔区RFLP分析，并将PCR检测和血清学检测的结果　进行比较。结果　表明：8个病人PCR检测结果　阳性，7个病人感染了B.garinii(第二基因种)、1个病人感染了B.afzelii(第三基因种)，同当地媒介生物中的优势基因种相同；血清学和PCR检测在对病人进行诊断时有互相弥补的作用。5s-23srRNA基因间隔区RFLP分析方法　有望在研究临床表现和基因种的关系及流行病学调查中得到广泛应用。
작자대래자모단강시임업의원화해림림장적34빈뇨액표본진행료5s-23srRNA기인간격구RFLP분석，병장PCR검측화혈청학검측적결과　진행비교。결과　표명：8개병인PCR검측결과　양성，7개병인감염료B.garinii(제이기인충)、1개병인감염료B.afzelii(제삼기인충)，동당지매개생물중적우세기인충상동；혈청학화PCR검측재대병인진행진단시유호상미보적작용。5s-23srRNA기인간격구RFLP분석방법　유망재연구림상표현화기인충적관계급류행병학조사중득도엄범응용。
The author tested 34 urine samples with 5s-23srRNA gene spacer RFLP analysis and compared PCR results with serodiagnosis results.8 pafients of urine are positive by PCR. 7 patients were infected by B. garinii, 1 patient by B afzelii. These genospecies infecting patients are identical with those infecting local vectors. The results showed that the accuracy of laboratory diagnosis oould be improved by simultaneously testing samples with PCR and serodiagnosis. The method of 5s-23srRNA gene spacer RFLP analysis will probably be used widely in studying the relationship of clinical manifestation and genospecies and in epidemiology investigations.