CAJ | 학술논문

对以霍乱毒素B亚基为载体蛋白的重组疟疾多价抗原在小鼠及恒河猴中的免疫原性及对相应疟原虫感染的免疫保护作用进行了研究。结果表明：该抗原免疫小鼠后，对约氏疟子孢子攻击的保护率在50％左右；恒河猴免疫后用1×108食蟹疟裂殖子攻击，对照组2只动物在攻击后4d感染，感染持续30d以上；免疫组2只动物中，两只动物在感染6～7d后完全恢复，且1只推迟3d感染，说明该抗原具有免疫保护作用。
대이곽란독소B아기위재체단백적중조학질다개항원재소서급항하후중적면역원성급대상응학원충감염적면역보호작용진행료연구。결과표명：해항원면역소서후，대약씨학자포자공격적보호솔재50％좌우；항하후면역후용1×108식해학렬식자공격，대조조2지동물재공격후4d감염，감염지속30d이상；면역조2지동물중，량지동물재감염6～7d후완전회복，차1지추지3d감염，설명해항원구유면역보호작용。
Recombinant fusion protein of cholera toxin B subunit (CTB) and poly-valent protective epitopes of plasmodium falciparum was given to i. m to C57BL/6j mice and rhesus monkeys three times. In rhesus monkeys, high level of antibodies for CTB (1:6400) and malaria epitopes (1:3200) amtobpdoes were elicited as well as the specific CTL activity for P. plasmodium. After the mice were challenged with sporozoites of P. yeolli, about 50 % of them were protected from the patent infection. A blood-stage challenge with 108 of P. cynomolgi parasite were given to rhesus monkeys, which showed that two animals in control group were patent infection for at least 30 days, in contrast, the two animals immunized were recovered respectively at the day of 11 and 15 after challenges. The results suggested that cholera toxin acts as an effective adjuvent in the development of malaria vaccine.