CAJ | 학술논문

目的研究1型糖尿病患者血清人类巨细胞病毒(HCMV)DNA与外周血T细胞亚群和胰岛功能的相关性.方法采用定量PCR技术测量20例1型糖尿病患者和40例对照组血清中HCMV DNA含量,并与外周血T细胞亚群、血糖(BG)、胰岛素(Ins)和C肽(C-P)水平进行比较和相关分析.结果1型糖尿病患者HCMV DNA阳性率及含量明显高于对照组.HCMV DNA阳性患者CD8、空腹及2h BG明显高于对照组,CD4、CD4/CD8比值、空腹及2h Ins和2h C-P明显低于对照组,HCMV DNA含量与CD8、空腹BG呈直线正相关,与CD4/CD8比值、空腹Ins及C-P呈直线负相关.而HCMV DNA阴性患者上述指标与对照组比较差异显著性不及阳性者.结论1型糖尿病患者HCMV感染与T细胞免疫和胰岛功能异常有关.
목적연구1형당뇨병환자혈청인류거세포병독(HCMV)DNA여외주혈T세포아군화이도공능적상관성.방법채용정량PCR기술측량20례1형당뇨병환자화40례대조조혈청중HCMV DNA함량,병여외주혈T세포아군、혈당(BG)、이도소(Ins)화C태(C-P)수평진행비교화상관분석.결과1형당뇨병환자HCMV DNA양성솔급함량명현고우대조조.HCMV DNA양성환자CD8、공복급2h BG명현고우대조조,CD4、CD4/CD8비치、공복급2h Ins화2h C-P명현저우대조조,HCMV DNA함량여CD8、공복BG정직선정상관,여CD4/CD8비치、공복Ins급C-P정직선부상관.이HCMV DNA음성환자상술지표여대조조비교차이현저성불급양성자.결론1형당뇨병환자HCMV감염여T세포면역화이도공능이상유관.
Objective To study the correlation between serum human cytomegalovirus (HCMV) DNA and peripheral T cell subsets as well as islet function in type 1 diabetic patients.Methods HCMV DNA levels in sera from 20 type 1 diabetic patients and 40 controls were measured by quantitative polymerase chain reaction (PCR),then the comparison and correlation analysis were made between HCMV DNA and T cell subsets,blood glucose (BG),insulin (Ins) and C peptide (C P).Results The positive rate and levels of HCMV DNA in type 1 diabetic patients were significantly higher than those of controls respectively.The percentage of CD 8 and the levels of fasting BG and BG 2 hours after meal in type 1 diabetic patients with positive HCMV DNA were by far higher than those in controls,while the percentage of CD 4,the ratio of CD 4/CD 8,the levels of fasting Ins,Ins and C P 2 hours after meal were significantly lower than those of controls.There existed the linear positive correlation between HCMV DNA and CD 8 or fasting BG,negative correlation between HCMV DNA and CD 4/CD 8,fasting Ins or fasting C P.Differences were not so significant between type 1 diabetic patients with negative HCMV DNA and controls compared with those between patients with positive HCMV DNA and controls.Conclusion HCMV infection is associated with dysfunction of islet and T cell mediated immunity in type 1 diabetic patients.