CAJ | 학술논문

[目的]对固始白鹅的遗传多样性进行微卫星标记分析.[方法]利用11个微卫星标记对固始白鹅进行遗传检测,筛选出多态性较好的微卫星位点,用变性聚丙烯酰胺凝胶电泳法分析这11 个微卫星位点在固始白鹅中的遗传变异,计算了微卫星位点的等位基因频率、杂合度、多态信息含量和有效等位基因数.[结果] 8个位点在固始白鹅上有较好的多态性,平均每个座位检测到4.25(3～6)个等位基因,平均杂合度为0.597(0.273～0.695),平均多态信息含量为0.635(0.530～0.769),平均有效等位基因数为3.344 7(2.383 0～4.984 0).[结论]为固始白鹅的保种和选育工作提供参考依据.
[목적]대고시백아적유전다양성진행미위성표기분석.[방법]이용11개미위성표기대고시백아진행유전검측,사선출다태성교호적미위성위점,용변성취병희선알응효전영법분석저11 개미위성위점재고시백아중적유전변이,계산료미위성위점적등위기인빈솔、잡합도、다태신식함량화유효등위기인수.[결과] 8개위점재고시백아상유교호적다태성,평균매개좌위검측도4.25(3～6)개등위기인,평균잡합도위0.597(0.273～0.695),평균다태신식함량위0.635(0.530～0.769),평균유효등위기인수위3.344 7(2.383 0～4.984 0).[결론]위고시백아적보충화선육공작제공삼고의거.
[Objective] The research aimed to analyze the genetic diversity of Gushi goose using microsatellite DNA markers. [Method] Relationships between 11 microsatellite loci in Gushi goose were analyzed by least squares linear model. Genetic variation of 11 microsatellites loci in Gushi goose was analyzed with denaturing polyacrylamide gel electrophoresis. Allele frequency, heterozygosity, polymorphic information content and the numbers of effective alleles of 11 microsatellite loci were calculated. [Result] The results indicated that 8 microsatellite markers were polymorphic with 4.25 on average. The average heterozygosity was 0.597, the average polymorphic information content was 0.635 and the average of effective alleles was 3.344 7. [Conclusion] The study can provide the reference for conservation and breeding.