CAJ | 학술논문

目的观察两种不同的血清视黄醇结合蛋白(RBP)试剂在同一台生化分析仪上的检测结果是否具有可比性.方法用两种试剂进行血清视黄醇结合蛋白测定,并对两种试剂的准确性、重复性、线性范围等结果进行了评价比较.结果金斯尔试剂与北加试剂比较,两种试剂间健康对照组回归方程Y=1.011X、r~2=0.960 4,患者组回归方程Y=1.043 8X、r~2=0.965 7,r~2＞0.95,测定高、低值质控品的相对偏差分别为-2.32%和-4.64%,其准确性偏差小于10%;高、低值质控品测定值的CV分别为0.9%和2.3%,患者标本测定值CV为1.3%,其重复性CV小于10%;在0.35～25 mg/Dl范围内,线性误差不超过10%.结论北京九强公司的金斯尔血清视黄醇结合蛋白检测试剂与上海北加试剂其检测结果具有可比性,可用于临床血清视黄醇结合蛋白的测定.
목적 관찰량충불동적혈청시황순결합단백(RBP)시제재동일태생화분석의상적검측결과시부구유가비성.방법 용량충시제진행혈청시황순결합단백측정,병대량충시제적준학성、중복성、선성범위등결과진행료평개비교.결과 금사이시제여북가시제비교,량충시제간건강대조조회귀방정Y=1.011X、r~2=0.960 4,환자조회귀방정Y=1.043 8X、r~2=0.965 7,r~2＞0.95,측정고、저치질공품적상대편차분별위-2.32%화-4.64%,기준학성편차소우10%;고、저치질공품측정치적CV분별위0.9%화2.3%,환자표본측정치CV위1.3%,기중복성CV소우10%;재0.35～25 mg/Dl범위내,선성오차불초과10%.결론 북경구강공사적금사이혈청시황순결합단백검측시제여상해북가시제기검측결과구유가비성,가용우림상혈청시황순결합단백적측정.
Objective The evaluation and comparison of two different kinds of serum retinol-binding protein(RBP)reagents in the same biochemical analyzer.Methods Use the reagents of Shanghai Beijia biochemical reagents company and Beijing Jiuqiang biochemical limited company for serum retinol-binding protein determination.Their accuracy,repeatability,sensitivity,linear range and interference are compared.Results The results of Gcell reagents and Beijia reagents were evaluated,which regression equation wasY=1.011X,r~2=0.9604 in the normal group and y=1.043 8X,r~2=0.9657 in the patient group;The CV%of high-level and low-level controls were 0.9%and 2.3 0A respectively.The CV% of patients was 1.3%,The results range in 0.35～25 mg/dL,the linear error was less than 10%.Conclusion The results of Gcell reagents and Shanghai Beijia reagents are comparable and Gcell reagents can be safely used in clinical analysis.