沈阳化工学院学报
瀋暘化工學院學報
침양화공학원학보
JOURNAL OF SHENYANG INSTITUTE OF CHEMICAL TECHNOLOGY
2010年
2期
97-103,159
,共8页
何玲玲%申华%刘瑕%郭虹%兰丽艳
何玲玲%申華%劉瑕%郭虹%蘭麗豔
하령령%신화%류하%곽홍%란려염
盐酸小檗碱%牛血清白蛋白%荧光光谱%紫外-可见光谱
鹽痠小檗堿%牛血清白蛋白%熒光光譜%紫外-可見光譜
염산소벽감%우혈청백단백%형광광보%자외-가견광보
berberine chloride%bovine serum albumin%fluorescence spectra.%UV-Vis spectra
采用荧光、同步荧光及紫外-可见光谱法研究溶液中盐酸小檗碱(BC)与牛血清白蛋白(BSA)的相互作用.结果表明:BC对BSA荧光有较强的猝灭作用,其猝灭机理为以静态猝灭为主并结合一定动态猝灭的复合过程.利用荧光猝灭反应求得BC与BSA之间的结合常数和结合位点数.根据Frster非辐射能量转移理论,计算得到BC与BSA之间的结合距离小于8 nm,表明二者之间能够发生能量转移.根据反应热力学参数确定二者的相互作用是一个自发的过程,且静电力是它们之间相互作用的主要作用力类型.采用同步荧光考察BC对BSA构象的影响,随着BC浓度的增大,色氨酸残基所处环境的疏水性改变,从而导致BSA的构象发生变化.
採用熒光、同步熒光及紫外-可見光譜法研究溶液中鹽痠小檗堿(BC)與牛血清白蛋白(BSA)的相互作用.結果錶明:BC對BSA熒光有較彊的猝滅作用,其猝滅機理為以靜態猝滅為主併結閤一定動態猝滅的複閤過程.利用熒光猝滅反應求得BC與BSA之間的結閤常數和結閤位點數.根據Frster非輻射能量轉移理論,計算得到BC與BSA之間的結閤距離小于8 nm,錶明二者之間能夠髮生能量轉移.根據反應熱力學參數確定二者的相互作用是一箇自髮的過程,且靜電力是它們之間相互作用的主要作用力類型.採用同步熒光攷察BC對BSA構象的影響,隨著BC濃度的增大,色氨痠殘基所處環境的疏水性改變,從而導緻BSA的構象髮生變化.
채용형광、동보형광급자외-가견광보법연구용액중염산소벽감(BC)여우혈청백단백(BSA)적상호작용.결과표명:BC대BSA형광유교강적졸멸작용,기졸멸궤리위이정태졸멸위주병결합일정동태졸멸적복합과정.이용형광졸멸반응구득BC여BSA지간적결합상수화결합위점수.근거Frster비복사능량전이이론,계산득도BC여BSA지간적결합거리소우8 nm,표명이자지간능구발생능량전이.근거반응열역학삼수학정이자적상호작용시일개자발적과정,차정전력시타문지간상호작용적주요작용력류형.채용동보형광고찰BC대BSA구상적영향,수착BC농도적증대,색안산잔기소처배경적소수성개변,종이도치BSA적구상발생변화.
The interaction between berberine chloride (BC) and bovine serum albumin (BSA) in aqueous solution was investigated by means of fluorescence,synchronous fluorescence and UV-Vis spectra.The fluorescence of BSA was quenched remarkably by BC and the quenching mechanism was deemed as mainly static quenching,accompanying with dynamic quenching at the same time.The binding constants K and the number of binding sites n were calculated at different temperatures by fluorescence quenching method.The BC-BSA binding distance was determined to be less than 8 nm according to Frster non-radioactive resonance energy transfer theory,and suggested that the energy transfer occured from BSA to BC.Based on the obtained thermodynamic parameters,the interaction process was spontaneous and the electrostatic forces played a major role in the process.In addition,the effect of BC on the conformation of BSA was analyzed by using synchronous fluorescence spectra.The alterative configuration of BSA was induced by the hydrophobic environment of tryptophan residues with increasing the BC concentration.