CAJ | 학술논문

目的建立一种检测人凝血因子制品中游离氨基酸含量的方法.方法采用茚三酮显色法结合三氯醋酸去蛋白质方法,利用分光光度计建立标准曲线,定量测定制品中的赖氨酸含量.结果用该方法绘制的标准曲线具有很好的相关性,相关系数(r)为0.9994±0.0004,线性范围为5.2～26.0 mg/L,试验内和试验间的变异系数分别为1.07%和3.06%,回收率为94.60%～97.60%.与氨基酸分析仪测定法比较,结果相近.结论本方法简便快速,灵敏度和准确度高,重复性好,适用于人凝血因子制品的生产质控.
목적 건립일충검측인응혈인자제품중유리안기산함량적방법.방법채용인삼동현색법결합삼록작산거단백질방법,이용분광광도계건립표준곡선,정량측정제품중적뢰안산함량.결과용해방법회제적표준곡선구유흔호적상관성,상관계수(r)위0.9994±0.0004,선성범위위5.2～26.0 mg/L,시험내화시험간적변이계수분별위1.07%화3.06%,회수솔위94.60%～97.60%.여안기산분석의측정법비교,결과상근.결론본방법간편쾌속,령민도화준학도고,중복성호,괄용우인응혈인자제품적생산질공.
Objective To establish a method for determination of the content of free amino acids in coagulation factor products. Methods Sample were deproteinised with 12% trichloroacetic acid precipi-tation. The deproteinised samples were reacted with ninhydrin to produce a colored substance. Finally,ly-sine in samples were determined quantitively by spectrophotometry. Results The calibration curve for the amino acid was linear (r=0.9994±0. 0004) in the range of 5.2-26.0 mg/L. The coefficients of varia-tion for intra-assay and inter-assay precision were 1.07 % and 3.06 % ,respectively. The recovery rate was 94.60%-97.60%. Conclusions This method is highly sensitive,accurate, with good reproducibility. It is simple and rapid and can be used for the quality control of human coagulation factor products.