国际医药卫生导报
國際醫藥衛生導報
국제의약위생도보
INTERNATIONAL MEDICINE & HEALTH GUIDANCE NEWS
2011年
13期
1619-1621
,共3页
反应曲线%生化分析仪%评价%室内质量控制
反應麯線%生化分析儀%評價%室內質量控製
반응곡선%생화분석의%평개%실내질량공제
Response curve%Biochemistry analyzer%Evaluation%Internal quality control
目的 利用深圳迈瑞BS-400生化分析仪(以下简称BS-400)反应曲线图,分析有关室内质量控制失控、标本测试结果异常以及评价试剂性能的问题.方法 观察、比较甘油三脂(TG)在控与失控反应曲线图,以及γ-谷氨酰基转移酶(GGT)、天门冬氨酸氨基转移酶(AST)等异常结果的反应曲线图,以TG测试为例,结合试剂说明书,观察反应曲线图,分析有关数据及图形.结果 室内质量控制中,TG试剂R1(以下简称R1)失效引起的失控,R1吸光度平均值8.39,正常平均值17,经t检验,和差异具有显著性(P＜0.05);测定标本时,GGT=-38 U/L,样本和R2孵育时的吸光度值超过37 331.AST=-25 U/L,曲线图形出现底物耗尽.这些异常结果的反应过程相关吸光度、图形、反应度(R)与正常反应曲线图的比较,有明显的不同;观察TG测试的反应曲线图,其试剂空白值(R0)=221.6、反应曲线平稳.无明显波动,反应4分钟后已趋于平衡,符合试剂说明书有关要求.结论 BS-400反应曲线图,可有效快速地分析有关室内质量控制失控、标本测试结果异常以及评价试剂性能的问题,为解决问题提供了一个极好的线索.
目的 利用深圳邁瑞BS-400生化分析儀(以下簡稱BS-400)反應麯線圖,分析有關室內質量控製失控、標本測試結果異常以及評價試劑性能的問題.方法 觀察、比較甘油三脂(TG)在控與失控反應麯線圖,以及γ-穀氨酰基轉移酶(GGT)、天門鼕氨痠氨基轉移酶(AST)等異常結果的反應麯線圖,以TG測試為例,結閤試劑說明書,觀察反應麯線圖,分析有關數據及圖形.結果 室內質量控製中,TG試劑R1(以下簡稱R1)失效引起的失控,R1吸光度平均值8.39,正常平均值17,經t檢驗,和差異具有顯著性(P＜0.05);測定標本時,GGT=-38 U/L,樣本和R2孵育時的吸光度值超過37 331.AST=-25 U/L,麯線圖形齣現底物耗儘.這些異常結果的反應過程相關吸光度、圖形、反應度(R)與正常反應麯線圖的比較,有明顯的不同;觀察TG測試的反應麯線圖,其試劑空白值(R0)=221.6、反應麯線平穩.無明顯波動,反應4分鐘後已趨于平衡,符閤試劑說明書有關要求.結論 BS-400反應麯線圖,可有效快速地分析有關室內質量控製失控、標本測試結果異常以及評價試劑性能的問題,為解決問題提供瞭一箇極好的線索.
목적 이용심수매서BS-400생화분석의(이하간칭BS-400)반응곡선도,분석유관실내질량공제실공、표본측시결과이상이급평개시제성능적문제.방법 관찰、비교감유삼지(TG)재공여실공반응곡선도,이급γ-곡안선기전이매(GGT)、천문동안산안기전이매(AST)등이상결과적반응곡선도,이TG측시위례,결합시제설명서,관찰반응곡선도,분석유관수거급도형.결과 실내질량공제중,TG시제R1(이하간칭R1)실효인기적실공,R1흡광도평균치8.39,정상평균치17,경t검험,화차이구유현저성(P＜0.05);측정표본시,GGT=-38 U/L,양본화R2부육시적흡광도치초과37 331.AST=-25 U/L,곡선도형출현저물모진.저사이상결과적반응과정상관흡광도、도형、반응도(R)여정상반응곡선도적비교,유명현적불동;관찰TG측시적반응곡선도,기시제공백치(R0)=221.6、반응곡선평은.무명현파동,반응4분종후이추우평형,부합시제설명서유관요구.결론 BS-400반응곡선도,가유효쾌속지분석유관실내질량공제실공、표본측시결과이상이급평개시제성능적문제,위해결문제제공료일개겁호적선색.
Objective To explore loss of internal quality control(IQC)and abnormal results of specimen tests, and to evaluate reagents property by using response curve on Mindray biochemistry analyzer BS-400. Methods The control and loss of control response cur e for triglyceride(TG), and the abnormal results of response curves for(a)-glutamyl transferase(GGT)and aspartate aminotransfera.se(AST)were observed and compared. Taking TG test as example and referring to reagent instruction manual, we analyzed relevant data and charts. Results In loss of IQC due to inefficacy of TG reagent(R1), the average OD value of R1 was 8.39, while the average normal value was 17, t- test showing a significant difference(P＜ 0.05). During the incubattion with R1, OD value of the sample exceeded 37 331 when GGT = -38 U/L. As AST = -25 U/L, the response curve resulted in substrate exhaustion. When a specimen test resulted in abnormal data, the OD value, response charts, and responsiveness were significantly different from those on the control response curves. The response curve of TG test was observed; the curve, whose agent blank value was equal to 221.6, was stable without significant fluctuation and it became balanced 4 min after reactions. The test results were in accordance with the reagent manual. Conclusions Response curve on Mindray biochemistry analyzer BS-400 is effective in analyzing loss of IOC and abnormal results of specimen tests and in evaluating reagents' property.
