中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2008年
12期
796-799
,共4页
庞莹%何春涤%刘勇%朱红%魏彬%王凯波%赵宁%王雅坤%肖汀%姜弈%魏华臣%陈洪铎
龐瑩%何春滌%劉勇%硃紅%魏彬%王凱波%趙寧%王雅坤%肖汀%薑弈%魏華臣%陳洪鐸
방형%하춘조%류용%주홍%위빈%왕개파%조저%왕아곤%초정%강혁%위화신%진홍탁
痤疮,寻常%受体,雄激素%重复序列,核酸%多态性,限制性片段长度
痤瘡,尋常%受體,雄激素%重複序列,覈痠%多態性,限製性片段長度
좌창,심상%수체,웅격소%중복서렬,핵산%다태성,한제성편단장도
Acne vulgaris%Receptors,androgen%Repetitive sequences,nucleic acid%Polymorphism,restriction fragment length
目的 探讨人雄激素受体基因第一外显子CAG重复序列长度多态性与痤疮发生之间的关系.方法 研究对象为中国东北地区238例痤疮患者和207例健康对照,抽取外周血后分离纯化出基因组DNA,采用微卫星扫描(STRs)方法分析CAG重复序列的多态性.结果 男性病例组和对照组的CAG重复均数分别为22.70±3.09和23.48±2.83,两组之间差异有统计学意义(P=0.046);将对照组中CAG重复次数的中位数23作为分割点分组比较,长/短CAG片段在男性病例和对照中的分布差异有统计学意义,携带有CAG短片段的男性较携带CAG长片段的男性患痤疮的风险性明显增加(OR值2.07;95%可信限为1.21～3.54).女性病例组和对照组的CAG重复均数分别为23.41±2.87和23.85±0.21,两组之间差异无统计学意义(P=0.115);按中位数分组比较长/短CAG片段在女性病例和对照中的分布差异有统计学意义,携带有CAG短片段的女性患痤疮的风险性明显增加(P=0.013,OR值2.05;95%可信限为1.18～3.56).结论 雄激素受体基因第一外显子CAG的重复次数与中国东北地区痤疮的发生有关,CAG重复次数少的男性个体患痤疮的风险性增加,雄激素受体基因第一外显子CAG的重复次数可作为痤疮的遗传易感标志之一.
目的 探討人雄激素受體基因第一外顯子CAG重複序列長度多態性與痤瘡髮生之間的關繫.方法 研究對象為中國東北地區238例痤瘡患者和207例健康對照,抽取外週血後分離純化齣基因組DNA,採用微衛星掃描(STRs)方法分析CAG重複序列的多態性.結果 男性病例組和對照組的CAG重複均數分彆為22.70±3.09和23.48±2.83,兩組之間差異有統計學意義(P=0.046);將對照組中CAG重複次數的中位數23作為分割點分組比較,長/短CAG片段在男性病例和對照中的分佈差異有統計學意義,攜帶有CAG短片段的男性較攜帶CAG長片段的男性患痤瘡的風險性明顯增加(OR值2.07;95%可信限為1.21～3.54).女性病例組和對照組的CAG重複均數分彆為23.41±2.87和23.85±0.21,兩組之間差異無統計學意義(P=0.115);按中位數分組比較長/短CAG片段在女性病例和對照中的分佈差異有統計學意義,攜帶有CAG短片段的女性患痤瘡的風險性明顯增加(P=0.013,OR值2.05;95%可信限為1.18～3.56).結論 雄激素受體基因第一外顯子CAG的重複次數與中國東北地區痤瘡的髮生有關,CAG重複次數少的男性箇體患痤瘡的風險性增加,雄激素受體基因第一外顯子CAG的重複次數可作為痤瘡的遺傳易感標誌之一.
목적 탐토인웅격소수체기인제일외현자CAG중복서렬장도다태성여좌창발생지간적관계.방법 연구대상위중국동북지구238례좌창환자화207례건강대조,추취외주혈후분리순화출기인조DNA,채용미위성소묘(STRs)방법분석CAG중복서렬적다태성.결과 남성병례조화대조조적CAG중복균수분별위22.70±3.09화23.48±2.83,량조지간차이유통계학의의(P=0.046);장대조조중CAG중복차수적중위수23작위분할점분조비교,장/단CAG편단재남성병례화대조중적분포차이유통계학의의,휴대유CAG단편단적남성교휴대CAG장편단적남성환좌창적풍험성명현증가(OR치2.07;95%가신한위1.21～3.54).녀성병례조화대조조적CAG중복균수분별위23.41±2.87화23.85±0.21,량조지간차이무통계학의의(P=0.115);안중위수분조비교장/단CAG편단재녀성병례화대조중적분포차이유통계학의의,휴대유CAG단편단적녀성환좌창적풍험성명현증가(P=0.013,OR치2.05;95%가신한위1.18～3.56).결론 웅격소수체기인제일외현자CAG적중복차수여중국동북지구좌창적발생유관,CAG중복차수소적남성개체환좌창적풍험성증가,웅격소수체기인제일외현자CAG적중복차수가작위좌창적유전역감표지지일.
Objective To investigate the relationship of CAG repeat length polymorphism in the androgen receptor(AR)gene to the development of acne.Methods A total of 238 patients with ache vulgaris and 207 healthy human controls in Northeast China were included in this study.Genomic DNA was isolated and purified from the blood of these subjects.The CAG repeat lengths in the AR gene were analyzed by somatic microsatellites (STRs).Results A significant difference was found in the CAG repeat number between the male acne Patients(22.70±3.09)and male controls(23.48±2.83,P=0.046),but not between the female cases and controls(23.41±2.87 versus 23.85±0.21.P=0.12).In order to assess the risk associated with CAG repeats,the male and female subjects were dichotomized based on the median repeat length in the corresponding control group as the arbitrary cut-off point.Those men and women with a short CAG repeat length(＜23 in men,and＜24 in women)had a significantly increased risk for agne than those with a long CAG repeat length(men:95%confidence interval,1.21-3.54,OR=2.07,P=0.008;women:95%confidence interval.1.18-3.56,OR=2.05,P=0.01).Conclusions This study strongly indicates that the CAG repeat length in AR gene is associated with the development of acne in Northeast China,and those men with a short CAG repeat length seem to have a high risk for ague.Consequently,CAG repeat length may serve as a genetic susceptibility marker.
