CAJ | 학술논문

目的研究血浆microRNA-22 (miR-22)在特发性肺动脉高压(IPAH)诊断中的价值及发病机制中的作用.方法应用real-time PCR方法检测IPAH患者及匹配健康人血浆miR-22表达水平,结合生物信息学方法分析miR-22靶基因MYCBP,并采用eGFP报告基因对靶基因进行实验验证.结果 IPAH患者血浆miR-22表达水平明显降低(P＜0.01),ROC曲线下面积(AUC)为0.744；生物信息学分析及eGFP报告基因验证,MYCBP是miR-22的靶基因之一.结论血浆miR-22在IPAH患者表达明显下调,可作为IPAH诊断分子标志物,miR-22通过靶基因MYCBP激活c-Myc信号通路,促进细胞增殖相关基因转录,是导致IPAH患者肺动脉中膜平滑肌增殖及肺血管重构的可能机制之一.
목적 연구혈장microRNA-22 (miR-22)재특발성폐동맥고압(IPAH)진단중적개치급발병궤제중적작용.방법 응용real-time PCR방법검측IPAH환자급필배건강인혈장miR-22표체수평,결합생물신식학방법분석miR-22파기인MYCBP,병채용eGFP보고기인대파기인진행실험험증.결과 IPAH환자혈장miR-22표체수평명현강저(P＜0.01),ROC곡선하면적(AUC)위0.744；생물신식학분석급eGFP보고기인험증,MYCBP시miR-22적파기인지일.결론 혈장miR-22재IPAH환자표체명현하조,가작위IPAH진단분자표지물,miR-22통과파기인MYCBP격활c-Myc신호통로,촉진세포증식상관기인전록,시도치IPAH환자폐동맥중막평활기증식급폐혈관중구적가능궤제지일.
Objective To investigate the value of plasma miR-22 in diagnosis of idiopathic pulmonary arterial hypertension ( IPAH ),and its role of regulation mechanisms in the pathogenesis of the disease.Methods Circulating miR-22 levels of IPAH patients and healthy controls were evaluated by RTPCR.The silico analysis of targets for miR-22 was taken, and followed by eGFP reporter assay for verification of predicted target gene Myc binding protein (MYCBP). Results Compared with healthy controls,the expression of plasma miR-22 in IPAH patients was significantly decreased (P ＜ 0.01 ).The area under curve (AUC) of ROC curve was 0.744.MYCBP was a real target of miR-22 confirmed by silico analysis and eGFP reporter assay. Conclusions The expression of plasma miR-22 was significantly decreased in IPAH patients,and it could serve as a potential biomarker for diagnosis.The miR-22 might be involved in the pathogenesis of the disease through promoting its target gene MYCBP to activate the c-Myc pathway.