CAJ | 학술논문

目的分析神经外科重症监护室白色念珠菌的药物敏感情况并建立随机扩增多态性DNA(RAPD)基因分型方法监测医院感染. 方法收集自2007年5月至11月从珠江医院神经外科重症监护室内分离的30株白色念珠菌,以微量液基稀释法分析常用抗真菌药物对白色念珠菌药物敏感情况,同时提取DNA后进行RAPD基因分型. 结果临床分离的30株白色念珠菌对抗真菌药物敏感性分别为伏立康唑(100%)、两性霉素B(100%)、氟康唑(96.3%)、氟胞嘧啶(93.0%)、伊曲康唑(90.0%).30株白色念珠菌RAPD基冈分型共分为27型,没有出现相同的带型. 结论在白色念珠菌感染的药物治疗中,伏立康唑、两性霉素B敏感性最好,氟康唑、氟胞嘧啶、伊曲康唑表现为较高的敏感性.我院神经外科重症监护室自2007年5月～11月期间并未出现白色念珠菌感染局部暴发流行.
목적 분석신경외과중증감호실백색념주균적약물민감정황병건립수궤확증다태성DNA(RAPD)기인분형방법 감측의원감염. 방법 수집자2007년5월지11월종주강의원신경외과중증감호실내분리적30주백색념주균,이미량액기희석법분석상용항진균약물대백색념주균약물민감정황,동시제취DNA후진행RAPD기인분형. 결과 림상분리적30주백색념주균대항진균약물민감성분별위복립강서(100%)、량성매소B(100%)、불강서(96.3%)、불포밀정(93.0%)、이곡강서(90.0%).30주백색념주균RAPD기강분형공분위27형,몰유출현상동적대형. 결론 재백색념주균감염적약물치료중,복립강서、량성매소B민감성최호,불강서、불포밀정、이곡강서표현위교고적민감성.아원신경외과중증감호실자2007년5월～11월기간병미출현백색념주균감염국부폭발류행.
Objective To analyze the drug resistance profile of the clinical isolates of Candida albicans and establish a randomly amplified polymorphic DNA (RAPD) assay for Candida albicans. Methods Thirty strains of Candida albicans were collected from the intensive care unit (ICU) of the department ofneurosurgery between May, 2007 and November, 2007. The drug resistance of Candida albicans was analyzed with liquid microdilution method, and the fungal DNA was extracted for genotyping by RAPD assay. Results The sensitivity of Candida albicans strains was 100% to the anti-fungal drug VRC, 100% to AMB (100%), 96.35% to FCZ, 93.0% to 5-FC, and 90.0% to ITR. The 30 Candida albicans strains were genotyped into 27 types with a typing rate of 90.0%. Conclusion AMB and VRC remain the primary options for treatment of Candida albicans infection, and the isolated Candida albicans strains are highly sensitive to FCZ, 5-FC, and ITR. No evidence has been identified to suggest a local outburst ofCandida albicans infection in our hospital between May, 2007 and November, 2007.