CAJ | 학술논문

目的探讨基质金属蛋白酶1/蛋白酶活性受体-1( MMP-1/PAR1)信号通路在脑胶质瘤发展的作用及其意义.方法应用免疫组织化学方法检测108例原发性胶质瘤患者肿瘤组织中MMP-1和PAR1的表达模式.采用Kaplan-Meier生存分析和Cox比例风险模型分析胶质瘤患者的预后.结果免疫染色结果显示108例患者活体组织有83.3％表达MMP-1 (90/108),76.9％表达PAR1(83/108).PAR1的表达与MMP-1的表达显著相关(r=0.8,P＜0.01).高级别胶质瘤MMP-1和PAR1的免疫组织化学(IHC)总分明显高于低级别胶质瘤(两者P＜0.01).此外,MMP-1和PAR1表达高的患者Kamofsky行为能力(KPS)评分低于MMP-1和PAR1表达低的患者(两者P＜0.01).而且,患者总体生存率降低的结果表明,MMP-1和PAR1表达情况是胶质瘤的强预后指标(P＜0.01).多因素Cox分析显示KPS (P ＜0.01)、WHO病理分级(P＜0.01)、MMP-1 (P＜0.01)和PAR1 (P ＜0.01)是影响脑胶质瘤预后的独立因素.结论脑胶质瘤中MMP-1/PAR1信号通路的活化与肿瘤恶性程度及临床结果相关.
목적 탐토기질금속단백매1/단백매활성수체-1( MMP-1/PAR1)신호통로재뇌효질류발전적작용급기의의.방법 응용면역조직화학방법검측108례원발성효질류환자종류조직중MMP-1화PAR1적표체모식.채용Kaplan-Meier생존분석화Cox비례풍험모형분석효질류환자적예후.결과 면역염색결과현시108례환자활체조직유83.3％표체MMP-1 (90/108),76.9％표체PAR1(83/108).PAR1적표체여MMP-1적표체현저상관(r=0.8,P＜0.01).고급별효질류MMP-1화PAR1적면역조직화학(IHC)총분명현고우저급별효질류(량자P＜0.01).차외,MMP-1화PAR1표체고적환자Kamofsky행위능력(KPS)평분저우MMP-1화PAR1표체저적환자(량자P＜0.01).이차,환자총체생존솔강저적결과표명,MMP-1화PAR1표체정황시효질류적강예후지표(P＜0.01).다인소Cox분석현시KPS (P ＜0.01)、WHO병리분급(P＜0.01)、MMP-1 (P＜0.01)화PAR1 (P ＜0.01)시영향뇌효질류예후적독립인소.결론 뇌효질류중MMP-1/PAR1신호통로적활화여종류악성정도급림상결과상관.
Objective To investigate the effect of matrix metalloproteinase-1 (MMP-1) and proteinase-activated receptor-1 (PAR1) on the progression of human gliomas.Methods Immunohistochemical staining was performed to detect the expression patterns of MMP-1 and PAR1 in biopsies from 108 patients with primary gliomas.Kaplan-Meier survival and Cox regression analyses were done to evaluate the prognosis of patients.Results Immunostaining revealed MMP-1 to be expressed in 83.3％ (90/108) and PAR1 in 76.9％ (83/108) of the biopsies respextively.PAR1 expression was significantly correlated with that of MMP-1 (r =0.8,P ＜ 0.01 ).The total immunohistochemistry (IHC) scores for MMP-1 and PAR1 were significantly higher in high-grade tumors than in low-grade tumors (both P ＜ 0.01 ).In addition,patients with high MMP-1 and high PAR1 expression had lower Kamofsky performance scale (KPS) scores than those with low MMP-1 and low PAR1 expression (both P ＜0.01 ).Moreover,MMP-1 and PAR1 expression was shown to be a strong prognostic marker for decreased overall survival (P ＜ 0.01,respectively).Furthermore,Cox multi-factor analysis showed that KPS ( P ＜ 0.01 ),WHO grade ( P ＜ 0.01 ),MMP-1 ( P ＜ 0.01 ),and PAR1 (P ＜ 0.01 ) were independent prognostic factors for human gliomas.Conclusion Our results suggest that in gliomas,the upregulation of MMP-1 and PAR1 correlates with histological malignancy grade and clinical outcome.