中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2010年
12期
1083-1086
,共4页
吴捷%刁保卫%周海建%朱建华%王多春%逄波%王瑞白%阚飙%王少玲%苏新元%马焱
吳捷%刁保衛%週海建%硃建華%王多春%逄波%王瑞白%闞飆%王少玲%囌新元%馬焱
오첩%조보위%주해건%주건화%왕다춘%방파%왕서백%감표%왕소령%소신원%마염
弧菌,霍乱%电泳,凝胶,脉冲场%核糖体基因型%聚类分析
弧菌,霍亂%電泳,凝膠,脈遲場%覈糖體基因型%聚類分析
호균,곽란%전영,응효,맥충장%핵당체기인형%취류분석
Vibrio cholerae%Electrophoresis,gel,pulsed-field%Ribotyping%Cluster analysis
目的 分析海南省2008年霍乱暴发菌株的分子分型成簇性,为疫情分析提供分离株的病原学特征,协助疫情判断.方法 从本次霍乱疫情患者中分离69株,外环境分离7株[其中患者家厕所1株,水样1株,患者家附近鱼(池)塘3株,患者上卫生所就诊时呕吐地面的涂抹拭子1株和海南大学食堂菜刀涂抹拭子1株]共76株,对分离株进行常规病原学检测,利用脉冲场凝胶电泳(PFGE)技术获取受试菌株的DNA分子指纹图谱并对其结果进行聚类分析,将PFGE图像应用BioNumerics(Version4.0)数据库软件(Applied Maths BVBA,Belium)进行处理,识别图像条带,自动生成得出相似系数.结果 2008年海南省暴发霍乱疫情中分离的76株霍乱菌株中,6月份分离到的5株分离菌株图谱相同,相似系数达100%;10-11月份自患者和环境水体中分离到的70株菌图谱完全一致,相似系数达100%,但与6月份分离株的图谱不同;1株在暴发中分离自食堂菜刀的菌株,其图谱与其他菌株差异较大,相似系数为79.7%.结论 海南2008年存在不同的霍乱暴发,10-11月出现在不同市县的疫情可能属于一起较大范围的流行,环境水体污染是传播的不可忽视的环节.
目的 分析海南省2008年霍亂暴髮菌株的分子分型成簇性,為疫情分析提供分離株的病原學特徵,協助疫情判斷.方法 從本次霍亂疫情患者中分離69株,外環境分離7株[其中患者傢廁所1株,水樣1株,患者傢附近魚(池)塘3株,患者上衛生所就診時嘔吐地麵的塗抹拭子1株和海南大學食堂菜刀塗抹拭子1株]共76株,對分離株進行常規病原學檢測,利用脈遲場凝膠電泳(PFGE)技術穫取受試菌株的DNA分子指紋圖譜併對其結果進行聚類分析,將PFGE圖像應用BioNumerics(Version4.0)數據庫軟件(Applied Maths BVBA,Belium)進行處理,識彆圖像條帶,自動生成得齣相似繫數.結果 2008年海南省暴髮霍亂疫情中分離的76株霍亂菌株中,6月份分離到的5株分離菌株圖譜相同,相似繫數達100%;10-11月份自患者和環境水體中分離到的70株菌圖譜完全一緻,相似繫數達100%,但與6月份分離株的圖譜不同;1株在暴髮中分離自食堂菜刀的菌株,其圖譜與其他菌株差異較大,相似繫數為79.7%.結論 海南2008年存在不同的霍亂暴髮,10-11月齣現在不同市縣的疫情可能屬于一起較大範圍的流行,環境水體汙染是傳播的不可忽視的環節.
목적 분석해남성2008년곽란폭발균주적분자분형성족성,위역정분석제공분리주적병원학특정,협조역정판단.방법 종본차곽란역정환자중분리69주,외배경분리7주[기중환자가측소1주,수양1주,환자가부근어(지)당3주,환자상위생소취진시구토지면적도말식자1주화해남대학식당채도도말식자1주]공76주,대분리주진행상규병원학검측,이용맥충장응효전영(PFGE)기술획취수시균주적DNA분자지문도보병대기결과진행취류분석,장PFGE도상응용BioNumerics(Version4.0)수거고연건(Applied Maths BVBA,Belium)진행처리,식별도상조대,자동생성득출상사계수.결과 2008년해남성폭발곽란역정중분리적76주곽란균주중,6월빈분리도적5주분리균주도보상동,상사계수체100%;10-11월빈자환자화배경수체중분리도적70주균도보완전일치,상사계수체100%,단여6월빈분리주적도보불동;1주재폭발중분리자식당채도적균주,기도보여기타균주차이교대,상사계수위79.7%.결론 해남2008년존재불동적곽란폭발,10-11월출현재불동시현적역정가능속우일기교대범위적류행,배경수체오염시전파적불가홀시적배절.
Objective To analyze the molecular characteristics and genetic correlations of vibrio cholerae islolates in Hainan in 2008, so as to provide pathogenic proof to diagnose the plague. Methods Seventy six cholera strains were isolated from this cholera epidemic. 69 strains were obtained from patients,7 were isolated from external environment, among which, one was from patient' s toilet, one from water sample,three were isolated from fish pond near patient's home,one came from swab of the patient vomit on the ground of health center and one from swab of kitchen knife from Hainan University canteen respectively. With conventional aetiological methods, pulse-field gel electrophoresis was conducted and the patterns of the 76 isolates were analyzed. The PFGE image was analyzed using BioNumerics (Version4. 0,Applied Maths BVBA, Belium). Image bands were identified and similarity coefficient was automatically generated. Results Seventy six strains were isolated from vibrio cholerae outbreaks in Hainan in 2008.5 PFGE patterns of patient's isolates in June were the same,sharing a similarity coefficient of 100%. 70 PFGE patterns of patients and water in October and November were completely same,the similarity coefficient being 100%. But they were not same as that of June. 1 PFGE pattern of isolate from the sample in Hainan University was different,only sharing a similarity coefficient of 79. 7% ,which showed no correlation with the outbreak. Conclusion Different outbreaks of vibrio cholera occurred in Hainan in 2008. The epidemic in October and November at different counties was one outbreak. The pollution of water in environment was an important factor for outbreak.
