CAJ | 학술논문

目的探讨持续弹性外牵引对小型猪乳头及其支撑组织微血管生成的影响,以阐明其矫正乳头内陷的作用机制.方法选用3个月龄雌性小型猪3只,每只均有12个乳头,共计36个乳头.每只猪留取4个乳头作为空白对照组,其余8个安放乳头内陷矫治器进行持续弹性外牵引为实验组,分别于牵引后2、4、8、12周切取每只小型猪的1个空白组乳头和2个实验组乳头,将切除的乳头标本分别行免疫组织化学染色观察血管内皮细胞生长因子(VEGF)、CD34在组织中的表达,统计其阳性细胞的百分比及微血管密度(MVD)的变化.结果实验组CD34、VEGF在2周表达增强,4周达最高峰,8、12周逐渐减弱,对照组表达较弱.VEGF阳性表达的积分吸光度值及MVD与对照组比较差异均有统计学意义(P＜0.01),二者之间呈显著正相关.结论持续弹性外牵引可促进乳头及其支撑组织VEGF及CD34表达和微血管生成,增加局部氧供给,从而使局部组织增生,是矫正乳头内陷的理论基础之一.
목적 탐토지속탄성외견인대소형저유두급기지탱조직미혈관생성적영향,이천명기교정유두내함적작용궤제.방법 선용3개월령자성소형저3지,매지균유12개유두,공계36개유두.매지저류취4개유두작위공백대조조,기여8개안방유두내함교치기진행지속탄성외견인위실험조,분별우견인후2、4、8、12주절취매지소형저적1개공백조유두화2개실험조유두,장절제적유두표본분별행면역조직화학염색관찰혈관내피세포생장인자(VEGF)、CD34재조직중적표체,통계기양성세포적백분비급미혈관밀도(MVD)적변화.결과 실험조CD34、VEGF재2주표체증강,4주체최고봉,8、12주축점감약,대조조표체교약.VEGF양성표체적적분흡광도치급MVD여대조조비교차이균유통계학의의(P＜0.01),이자지간정현저정상관.결론 지속탄성외견인가촉진유두급기지탱조직VEGF급CD34표체화미혈관생성,증가국부양공급,종이사국부조직증생,시교정유두내함적이론기출지일.
Objective To explore the effect of elastic outside distraction on microvessel density and to illustrate the mechanism correcting inverted nipples. Methods Three female 3-month-old minipigs,with 12 nipples each,were employed.4 nipples of each minipig were remained without distraction were control group,and the other 8 nipples were continuously distracted with inverted nipple correction instruments.Corresponding nipples were excised at 2,4,8 and 12 weeks after traction.The excised nipple specimens were employed with immunohistochemical staining to detect the expression of vascular endothelial cells growth factor (VEGF) and CD34 in those tissues,and the percentageof positive cells and microvessel density (MVD) changes were counted.Results The expression of CD34 and VEGF in the experimental groups increased at 2 weeks after tracted,and reached at the peak at 4 weeks,then gradually decreased during 8 to 12 weeks after tracted.While those were weakly positive in control group.Compared with the control group,the integral optical density of VEGF-positive cells and MVD were significantly different (P＜0.01),and there was a significantly positive correlation between MVD and VEGF.Conclusions Continuous elastic outside distraction can promote the expression of VEGF and CD34 in female minipig nipple and enhance angiogenesis,which increases oxygen supply locally,and therefore those local tissue proliferation may be one of key mechanisms of correcting the inverted nipple by continuous elastic outside distraction.