CAJ | 학술논문

目的研究原发性肝癌患者与健康人血清蛋白质表达的差异性,寻找对原发性肝癌更有效的肿瘤诊断标志物.方法应用表面增强激光解析电离飞行时间质谱(SELDI-TOF-MS)技术榆测50例原发性肝癌患者、50名健康对照者血清中的蛋白质谱,寻找有意义的蛋白质谱.同时采用双抗体夹心法检测血清甲胎蛋白(AFP).结果原发性肝癌患者血清4个蛋白质峰与健康对照组比较差异有统计学意义.分别为:3354.71,8825.80(高表达),4345.08,13 715.01(低表达).从中筛选出质荷比(M/Z)分别为3354.71、8825.80差别最显著的蛋白质峰,使用这两个蛋白质峰作为原发性肝癌的诊断模式,其灵敏度、特异度分别为90%(45/50),94%(47/50).100例患者中,AFP阳性27例,灵敏度为54%(27/50),特异度为100%(30/30).结论 SELDI-TOF-MS蛋门质芯片技术在原发性肝癌的诊断及肿瘤特异性蛋白质生物标志的筛选中具有一定的价值,灵敏度和特异度较高,操作简单,正确诊断指数(r=83)优于AFP(r=53).
목적 연구원발성간암환자여건강인혈청단백질표체적차이성,심조대원발성간암경유효적종류진단표지물.방법 응용표면증강격광해석전리비행시간질보(SELDI-TOF-MS)기술유측50례원발성간암환자、50명건강대조자혈청중적단백질보,심조유의의적단백질보.동시채용쌍항체협심법검측혈청갑태단백(AFP).결과 원발성간암환자혈청4개단백질봉여건강대조조비교차이유통계학의의.분별위:3354.71,8825.80(고표체),4345.08,13 715.01(저표체).종중사선출질하비(M/Z)분별위3354.71、8825.80차별최현저적단백질봉,사용저량개단백질봉작위원발성간암적진단모식,기령민도、특이도분별위90%(45/50),94%(47/50).100례환자중,AFP양성27례,령민도위54%(27/50),특이도위100%(30/30).결론 SELDI-TOF-MS단문질심편기술재원발성간암적진단급종류특이성단백질생물표지적사선중구유일정적개치,령민도화특이도교고,조작간단,정학진단지수(r=83)우우AFP(r=53).
Objective To explore the tumor markers for the diagnosis of primary liver carcinomas (PLC) by detecting the serum protein spectrum differently expressed between PLC patients and healthy controls. Methods We detected the serum protein spectrum in 50 PLC patients and 50 healthy controls using surface enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF-MS) technique and find the significant protein peaks. The serum Alpha-fetoprotein (AFP) levels in all 100 serum samples were also measured by ELISA. Results The protein peaks, which could discriminate healthy individuals from PLC patients, were detected. Four protein molecules (3354.71, 8825.80, 4345.08, 13 715.01) had a significant difference between PLC patients and the normal controls (P <10-5), indicating that these protein molecules might be a potential marker for PLC. The specificity and sensitivity of SELDI-TOF-MS were 94% and 90% respectively. Sixteen PLC patients were AFP positive and the sensitivity was 54%(27/50). Conclusion With a high specificity and sensitivity, the detection of serum protein spectrum can be performed easily and quickly by SELDI-TOF-MS technique, which provides a serological way in identifying PLC and most likely to benefit from AFP strategies.