中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2008年
10期
725-728
,共4页
石琳熙%陆融%王莉%周春雷%徐琼%朱志峰%马睿%张华俐%贾静%傅正%姚智
石琳熙%陸融%王莉%週春雷%徐瓊%硃誌峰%馬睿%張華俐%賈靜%傅正%姚智
석림희%륙융%왕리%주춘뢰%서경%주지봉%마예%장화리%가정%부정%요지
三肽化合物酪丝缬肽%肝细胞性肝癌%中空纤维法
三肽化閤物酪絲纈肽%肝細胞性肝癌%中空纖維法
삼태화합물락사힐태%간세포성간암%중공섬유법
Tyroservatide%Hepatocellular carcinoma%Hollow fiber assay
目的 观察三肽化合物酪丝缬肽(YSV)的抗肝癌作用.方法 应用新型四氮唑盐(MTS)法,检测YSV对BEL-7402、SMMIC-7721、Hep3B、Her,G2和SK-HEP-1人肝癌细胞体外生长的抑制作用;应用中空纤维管皮下移植模型,进一步观察YSV对这5种人肝癌细胞体内生长的影响.结果 0.1～1.6 mg/ml的YSV能够明显抑制BEL-7402、SMMC-7721、Hep3B、ItepG2和SK-HEP-1人肝癌细胞的体外生长,YSV组的吸光度(A)值与相应阴性对照组相比,差异均有统计学意义(P<0.05),其中YSV对人肝癌细胞BEL-7402的抑制作用最强,1.6 mg/ml的YSV抑制率达63.3%.160μg·kg-1·d-1和320 μg·kg-1·d-1的YSV均能够明显抑制BEL-7402、SMMC-7721、ttep3B、HepG2和SK-HEP-1人肝癌细胞的体内生长,YSV组的A值与相应生理盐水组相比,差异均有统计学意义(P<0.05).YSV对人肝癌细胞BEL-7402的抑制作用最强,320 μg·kg-1·-1 YSV的抑制率为53.1%.结论 中空纤维法可以快速、准确地观察药物对不同肝癌细胞株体内生长的抑制作用,为YSV的临床应用提供了实验指导和可靠证据.
目的 觀察三肽化閤物酪絲纈肽(YSV)的抗肝癌作用.方法 應用新型四氮唑鹽(MTS)法,檢測YSV對BEL-7402、SMMIC-7721、Hep3B、Her,G2和SK-HEP-1人肝癌細胞體外生長的抑製作用;應用中空纖維管皮下移植模型,進一步觀察YSV對這5種人肝癌細胞體內生長的影響.結果 0.1～1.6 mg/ml的YSV能夠明顯抑製BEL-7402、SMMC-7721、Hep3B、ItepG2和SK-HEP-1人肝癌細胞的體外生長,YSV組的吸光度(A)值與相應陰性對照組相比,差異均有統計學意義(P<0.05),其中YSV對人肝癌細胞BEL-7402的抑製作用最彊,1.6 mg/ml的YSV抑製率達63.3%.160μg·kg-1·d-1和320 μg·kg-1·d-1的YSV均能夠明顯抑製BEL-7402、SMMC-7721、ttep3B、HepG2和SK-HEP-1人肝癌細胞的體內生長,YSV組的A值與相應生理鹽水組相比,差異均有統計學意義(P<0.05).YSV對人肝癌細胞BEL-7402的抑製作用最彊,320 μg·kg-1·-1 YSV的抑製率為53.1%.結論 中空纖維法可以快速、準確地觀察藥物對不同肝癌細胞株體內生長的抑製作用,為YSV的臨床應用提供瞭實驗指導和可靠證據.
목적 관찰삼태화합물락사힐태(YSV)적항간암작용.방법 응용신형사담서염(MTS)법,검측YSV대BEL-7402、SMMIC-7721、Hep3B、Her,G2화SK-HEP-1인간암세포체외생장적억제작용;응용중공섬유관피하이식모형,진일보관찰YSV대저5충인간암세포체내생장적영향.결과 0.1～1.6 mg/ml적YSV능구명현억제BEL-7402、SMMC-7721、Hep3B、ItepG2화SK-HEP-1인간암세포적체외생장,YSV조적흡광도(A)치여상응음성대조조상비,차이균유통계학의의(P<0.05),기중YSV대인간암세포BEL-7402적억제작용최강,1.6 mg/ml적YSV억제솔체63.3%.160μg·kg-1·d-1화320 μg·kg-1·d-1적YSV균능구명현억제BEL-7402、SMMC-7721、ttep3B、HepG2화SK-HEP-1인간암세포적체내생장,YSV조적A치여상응생리염수조상비,차이균유통계학의의(P<0.05).YSV대인간암세포BEL-7402적억제작용최강,320 μg·kg-1·-1 YSV적억제솔위53.1%.결론 중공섬유법가이쾌속、준학지관찰약물대불동간암세포주체내생장적억제작용,위YSV적림상응용제공료실험지도화가고증거.
Objective To investigate the inhibitory effect of tyroservatide (YSV) on growth of hepatocellular carcinoma cells. Methods In vitro effects of YSV on five human hepatocellular carcinoma cell lines were assayed by MTS. In vivo effects of YSV on 5 human hepatocellular carcinoma cell lines were assayed by hollow fiber tumor model. Results After treatment with YSV at a dose of 0.1～1.6 mg/ml, the growth of the five cell lines was significantly inhibited in vitro compared with that of the control group (P< 0.05 ). Especially, YSL remarkably inhibited the growth of human hepatocellular carcinoma BEL-7402 cells, i.e. the cell growth was inhibited by 63.3% after treatment with YSL at 1.6 mg/ml. The hollow fiber tumor model demonstrated that YSL (320 μg· kg-1·d-1 and 640 μg·kg-1·d-1 ) treatment significantly inhibited the in vivo growth of the five cancer cell lines compared with that in the saline control (P<0.05 ).YSL showed the highest level of inhibition of human BEL-7402 hepatocellular carcinoma cells, with an inhibitory index of 53.1% at320 μg·kg-1·d-1. Condusion As a new method, hoUow fiber assay may be used to evaluate the inhibitory effect of drugs on different tumor cells in vivo, rapidly, accurately and economically. Our results provide an instruction and evidence for clinical use of YSV.
