CAJ | 학술논문

本文首次利用核酸二级结构特征代替核酸碱基作为探讨类群之间亲缘关系的信号,构建了基于结构特征的子囊菌部分类群的系统进化树.该方法以S(规范的碱基对),Q(不规范的碱基对),I(单链),B(侧环),M(多分枝环)和H(发卡结构)为代码将二级结构特征区分为6种不同的亚结构类型,然后将二级结构特征转换为结构序列,并进行结构序列分析.该方法使rRNA不只局限于碱基比较,拓展了其应用范围,为揭示分子的功能与进化的关系提供了线索.结果表明,结构序列分析可用于子囊菌的系统学研究;相对于核酸序列分析,结构分析的结果似乎更加清晰地体现子囊果的演化过程.
본문수차이용핵산이급결구특정대체핵산감기작위탐토류군지간친연관계적신호,구건료기우결구특정적자낭균부분류군적계통진화수.해방법이S(규범적감기대),Q(불규범적감기대),I(단련),B(측배),M(다분지배)화H(발잡결구)위대마장이급결구특정구분위6충불동적아결구류형,연후장이급결구특정전환위결구서렬,병진행결구서렬분석.해방법사rRNA불지국한우감기비교,탁전료기응용범위,위게시분자적공능여진화적관계제공료선색.결과표명,결구서렬분석가용우자낭균적계통학연구;상대우핵산서렬분석,결구분석적결과사호경가청석지체현자낭과적연화과정.
A new approach using sequences of secondary structure to assess phylogenetic relationships is introduced. The descriptive dimensional positions of bases instead of sequences of nucleic acids are used as specific characters to construct a phylogenetic tree. Application of the method to phylogenetic analysis of selected taxa of Ascomycota at different taxonomic ranks indicates that structure signal is useful for exploring the phylogenetic relationships among groups. As a proof,the nucleotide signal with the same informative sites was also performed. Comparison between the structure trees and nucleotide trees revealed that structure analysis gives stronger support to classification based on morphological features and to phylogeny established by RPB2 sequence analysis. The method extends the application of rRNA molecule,which makes analysis based on rRNA sequences not limited to base comparison and is complementary to primary sequence analysis in fungal phylogenetic studies.