CAJ | 학술논문

[目的]研究酯和内酯的分光光度检测方法.[方法]首先,酯和内酯在碱性条件下与羟胺反应生成异羟肟酸;然后,在酸性条件下异羟肟酸又与Fe~(3+) 络合生成紫红色的异羟肟酸铁络合物;最后,在506 nm波长下间接测定酯和内酯含量.[结果]相同浓度的酯或内酯,与其相应的络合物吸光度相同,且络合物吸光度与其相应的酯或内酯的浓度呈良好的线性关系,标准曲线回归方程为y=4.118 6 x + 0.006 7 (R~2=0.999 6),平均回收率为102.5%,相对标准偏差RSD为0.64%.[结论]分光光度法具有较高的灵敏度、准确度和精密度,适用于各种酯或内酯及混合酯总量的测定.
[목적]연구지화내지적분광광도검측방법.[방법]수선,지화내지재감성조건하여간알반응생성이간우산;연후,재산성조건하이간우산우여Fe~(3+) 락합생성자홍색적이간우산철락합물;최후,재506 nm파장하간접측정지화내지함량.[결과]상동농도적지혹내지,여기상응적락합물흡광도상동,차락합물흡광도여기상응적지혹내지적농도정량호적선성관계,표준곡선회귀방정위y=4.118 6 x + 0.006 7 (R~2=0.999 6),평균회수솔위102.5%,상대표준편차RSD위0.64%.[결론]분광광도법구유교고적령민도、준학도화정밀도,괄용우각충지혹내지급혼합지총량적측정.
[Objective]The aim of this research was to study the detection method of ester and lactone by spectrophotometry.[Method]First of all,esters and lactone reacted with hydroxylamine and formed hydroxamic acid under alkaline conditions;secondly,hydroxamic acid reacted with Fe~(3+) and generated amaranthine hydroxamic acid-iron complex;finally,esters and lactone contents were measured indirectly at the wavelength of 506 nm.[Result]The results were found that the absorbance of complex was invariable in the same concentration of ester or lactone and had a good linear relationship.The standard curve regression equation was y=4.118 6 x+0.006 7(R~2=0.999 6) and the average recovery and relative standard deviation (RSD) were 102.5% and 0.64%,respectively.[Conclusion]The method has higher sensitivity,accuracy and precision,could be applied to determine the contents of carboxylic ester,lactone and their complexes.