CAJ | 학술논문

根据已报道的番茄Pto基因家族成员之一LescPtoF基因的序列特征设计引物,通过PCR扩增从3个感病番茄品种中获得3个同源序列.Blast结果表明其中两个序列和报道基因完全一致,另外一个和报道基因的序列相似性为99%,表明感病番茄品种中也存有LescPtoF基因的同源序列.
근거이보도적번가Pto기인가족성원지일LescPtoF기인적서렬특정설계인물,통과PCR확증종3개감병번가품충중획득3개동원서렬.Blast결과표명기중량개서렬화보도기인완전일치,령외일개화보도기인적서렬상사성위99%,표명감병번가품충중야존유LescPtoF기인적동원서렬.
Two specific primers were synthesized based on the published sequence of the tomato gene LescPtoF.Three sequences were determined for three susceptible tomato varieties by PCR.Blast results showed two of them had the same sequences as the reported classical LescPtoF,and its identity with other sequences was 99%.All these indicated that the susceptible tomato varieties might have the homologous sequence of the resistance geue.