CAJ | 학술논문

为建立H5N1亚型人源禽流感病毒A/Anhui/2/2005(W-AH05)反向遗传操作系统,本研究构建了W-AH05的8重组质粒,拯救出人源禽流感病毒R-A/Anhui/2/2005(R-AH05).全基因序列测定结果表明,救获病毒R-AH05与野生病毒W-AH05的核苷酸序列完全一致;动物试验证实,R-AH05保持了W-AH05的对BALB/c小鼠呈高致病力的特性,其MLD_(50)分别为1.5 log10EID_(50)和1.2 log10EID_(50).R-AH0与W-AH05分别以10_6 EID_(50)剂量鼻9腔感染BALB/c小鼠,病毒在小鼠体内的分布情况及各个脏器中的病毒滴度基本相同.由此可见,R-AH05保持了W-AH05的生物学特性,从而为进一步研究人源禽流感病毒的致病机理及跨宿主传播机制等提供了操作平台.
위건립H5N1아형인원금류감병독A/Anhui/2/2005(W-AH05)반향유전조작계통,본연구구건료W-AH05적8중조질립,증구출인원금류감병독R-A/Anhui/2/2005(R-AH05).전기인서렬측정결과표명,구획병독R-AH05여야생병독W-AH05적핵감산서렬완전일치;동물시험증실,R-AH05보지료W-AH05적대BALB/c소서정고치병력적특성,기MLD_(50)분별위1.5 log10EID_(50)화1.2 log10EID_(50).R-AH0여W-AH05분별이10_6 EID_(50)제량비9강감염BALB/c소서,병독재소서체내적분포정황급각개장기중적병독적도기본상동.유차가견,R-AH05보지료W-AH05적생물학특성,종이위진일보연구인원금류감병독적치병궤리급과숙주전파궤제등제공료조작평태.
To obtain the rescued virus of the H5N1 avian influenza virus from human isolate A/Anhui/2/2005 (W-AH05), eight plasmid system was established, and the rescued virus R-AH05 was generated by reverse genetic technique. The full genome of the R-AH05 had no nucleic acid change, compared with W-AH05 through sequence analysis. R-AH05 had the same pathogenicity as the parent virus in mice with 50 % mouse lethal dose (MLD_(50)) of 1.5 log10E1D_(50) and 1.2 log10EID_(50), respectively. The tissue tropism and virus load of R-AH05 and W-AH05 were highly identical in respective tissues of the mice infected intranasally with 10~6 EID_(50) dose. In conclusion, the rescued virus R-AH05 had congruously biological characteristics with the wild type virus W-AH05. The results could provide a technique for further research on the pathogenic and crossing-host-barrier mechanisms of the H5N1 avian influenza virus from human isolate.