中国血吸虫病防治杂志
中國血吸蟲病防治雜誌
중국혈흡충병방치잡지
CHINESE JOURNAL OF SCHISTOSOMIASIS CONTROL
2010年
1期
59-62
,共4页
旋毛虫%成囊前期幼虫%虫体抗原%排泄分泌抗原%表面抗原%保护性免疫
鏇毛蟲%成囊前期幼蟲%蟲體抗原%排洩分泌抗原%錶麵抗原%保護性免疫
선모충%성낭전기유충%충체항원%배설분비항원%표면항원%보호성면역
Trichinella spiralis%Encapsulated larva%Somatic antigen%Excretory-secretory antigen%Surface antigen%Protective immuni押
目的 比较旋毛虫成囊前期幼虫虫体抗原、排泄分泌抗原和表面抗原对小鼠产生的免疫保护作用.方法 分 .别用旋毛虫成囊前期幼虫虫体抗原、排泄分泌抗原、表面抗原免疫小鼠,同时设佐剂组和阴性对照组,间隔7 d免疫1次,共3次.末次免疫后7 d,每只小鼠用200条旋毛虫感染期幼虫经口进行攻击感染.感染后7 d和30 d分别检查各组小鼠肠道成虫数和肌幼虫数;用ELISA测血清中抗旋毛虫肌幼虫IgG抗体.结果 虫体抗原、排泄分泌抗原、表面抗原和佐剂组的成虫减虫率分别为84.89%、89.73%、85.65%、2.57%;肌幼虫减虫率分别为71.71%、80.98%、73.66%、5.60%.排泄分泌抗原组、表面抗原组的成虫减虫率(P均<0.05)及肌幼虫减虫率(P均<0.01)均高于虫体抗原组.各免疫组小鼠血清IgG抗体滴度明显升高,虫体抗原组、排泄分泌抗原组和表面抗原组的几何平均倒数滴度分别为2 798.89、3 474.51、2 984.83,分别为阴性对照组(459.32)的6.09、7.56、6.50倍.结论 旋毛虫成囊前期幼虫虫体抗原、排泄分泌抗原和表面抗原均能诱导宿主产生较强的抗攻击感染保护力.成囊前期幼虫的排泄分泌抗原显示出更强的免疫原性.
目的 比較鏇毛蟲成囊前期幼蟲蟲體抗原、排洩分泌抗原和錶麵抗原對小鼠產生的免疫保護作用.方法 分 .彆用鏇毛蟲成囊前期幼蟲蟲體抗原、排洩分泌抗原、錶麵抗原免疫小鼠,同時設佐劑組和陰性對照組,間隔7 d免疫1次,共3次.末次免疫後7 d,每隻小鼠用200條鏇毛蟲感染期幼蟲經口進行攻擊感染.感染後7 d和30 d分彆檢查各組小鼠腸道成蟲數和肌幼蟲數;用ELISA測血清中抗鏇毛蟲肌幼蟲IgG抗體.結果 蟲體抗原、排洩分泌抗原、錶麵抗原和佐劑組的成蟲減蟲率分彆為84.89%、89.73%、85.65%、2.57%;肌幼蟲減蟲率分彆為71.71%、80.98%、73.66%、5.60%.排洩分泌抗原組、錶麵抗原組的成蟲減蟲率(P均<0.05)及肌幼蟲減蟲率(P均<0.01)均高于蟲體抗原組.各免疫組小鼠血清IgG抗體滴度明顯升高,蟲體抗原組、排洩分泌抗原組和錶麵抗原組的幾何平均倒數滴度分彆為2 798.89、3 474.51、2 984.83,分彆為陰性對照組(459.32)的6.09、7.56、6.50倍.結論 鏇毛蟲成囊前期幼蟲蟲體抗原、排洩分泌抗原和錶麵抗原均能誘導宿主產生較彊的抗攻擊感染保護力.成囊前期幼蟲的排洩分泌抗原顯示齣更彊的免疫原性.
목적 비교선모충성낭전기유충충체항원、배설분비항원화표면항원대소서산생적면역보호작용.방법 분 .별용선모충성낭전기유충충체항원、배설분비항원、표면항원면역소서,동시설좌제조화음성대조조,간격7 d면역1차,공3차.말차면역후7 d,매지소서용200조선모충감염기유충경구진행공격감염.감염후7 d화30 d분별검사각조소서장도성충수화기유충수;용ELISA측혈청중항선모충기유충IgG항체.결과 충체항원、배설분비항원、표면항원화좌제조적성충감충솔분별위84.89%、89.73%、85.65%、2.57%;기유충감충솔분별위71.71%、80.98%、73.66%、5.60%.배설분비항원조、표면항원조적성충감충솔(P균<0.05)급기유충감충솔(P균<0.01)균고우충체항원조.각면역조소서혈청IgG항체적도명현승고,충체항원조、배설분비항원조화표면항원조적궤하평균도수적도분별위2 798.89、3 474.51、2 984.83,분별위음성대조조(459.32)적6.09、7.56、6.50배.결론 선모충성낭전기유충충체항원、배설분비항원화표면항원균능유도숙주산생교강적항공격감염보호력.성낭전기유충적배설분비항원현시출경강적면역원성.
Objective To compare the immune protective effects of three antigens of Trichinella spiralis encapsulated larvae on mice.Methods The mice were immunized with Trichinella spiralis encapsulated larvae somatic antigen,encapsulated larva excretory-secretory antigen and encapsulated larva surface antigen,3 times with a 7-day interval,and the adjuvant control and normal control group were set up.Seven days after the final immunization,each mouse was orally challenged with 200 Trichinella spirais larvae.The intestinal adult worms and muscle larvae of Trichinella spiralis of each group were recoveried and examined on Day 7 and Day 30 post-challenge,respectively.The level of 8eruln IgG to antigens of Trichinella muscle muscle larvae wa8 detected by ELISA.Results The intestinal adult worms were reduced by 84.89%.89.73%,85.65%.2.57% in the encapsulated larva somatic,excretory-secretory and surface antigen groups,respectively.The muscle lalwae were reduced by 71.71%,80.98%,73.66%,5.60%, respectively.Adtlltwornl reduction rates(P<0.05) and musclelarva reduction rates(P<0.01) of the encapsulated larva excretory-secretory antigen group and surface antigen group were higher than those of encapsulated larva somatic antigen group.The antibody titers in all the immunized groups increased significantly.and the GMRT values of the encapsulated larva somatic,excretory-secretory and surface antigen groups were 32 798.89,3 474.51,2 984.83,respectively,and were 6.09,7.56,6.50 times higher than those of the normal control group(459.32).Conclusions Trichinella spiralis encapsulated larva antigens can induce strong resistance of host to a subsequent challenge infection.Among these antigens,excretory-secretory antigen is more immunogenic.
