CAJ | 학술논문

为了研究刺五加提取物(ASE)替代抗生素降低仔猪早期断奶应激的效果及其作用机制.刺五加提取物多糖、黄酮和有机酸含量分别为2.94,0.19和1.04%;试验选用96头体重相近的21日龄杜×长×大断奶仔猪,按体重和性别随机分为3个处理,每个处理4个重复,分别饲喂基础日粮、添加ASE日粮(1 g/kg)和添加抗生素日粮(0.04%杆菌肽锌+0.03%咔吧氧).试验期为14 d,观察仔猪生长性能,测定7和14 d血清抗氧化指标等的变化.与基础日粮组相比,ASE和抗生素分别提高了仔猪日增重(ADG)16.81(P＞0.05)和24.66%(P＜0.05),均显著降低了料肉比(F/G)(P＜0.05);与抗生素组差异不显著.ASE和抗生素均可提高血清SOD水平;但是,在抗生素组中,第14 d仔猪血清SOD有降低的趋势(从147.96±15.88 U/mL降低至125.68±14.26U/mL).ASE有提高断奶后第7和14 d血清GSH含量的趋势(P=0.235和0.211),并极显著降低了7和14 d血清MDA水平(P＜0.05).结果表明,ASE可促进仔猪生长,提高机体抗氧化能力,在一定程度上可以替代抗生素.
위료연구자오가제취물(ASE)체대항생소강저자저조기단내응격적효과급기작용궤제.자오가제취물다당、황동화유궤산함량분별위2.94,0.19화1.04%;시험선용96두체중상근적21일령두×장×대단내자저,안체중화성별수궤분위3개처리,매개처리4개중복,분별사위기출일량、첨가ASE일량(1 g/kg)화첨가항생소일량(0.04%간균태자+0.03%잡파양).시험기위14 d,관찰자저생장성능,측정7화14 d혈청항양화지표등적변화.여기출일량조상비,ASE화항생소분별제고료자저일증중(ADG)16.81(P＞0.05)화24.66%(P＜0.05),균현저강저료료육비(F/G)(P＜0.05);여항생소조차이불현저.ASE화항생소균가제고혈청SOD수평;단시,재항생소조중,제14 d자저혈청SOD유강저적추세(종147.96±15.88 U/mL강저지125.68±14.26U/mL).ASE유제고단내후제7화14 d혈청GSH함량적추세(P=0.235화0.211),병겁현저강저료7화14 d혈청MDA수평(P＜0.05).결과표명,ASE가촉진자저생장,제고궤체항양화능력,재일정정도상가이체대항생소.
This study was conducted to investigate the effect of Acanthopanax senticosus extract(XSE)on the growth performance and antioxidant capacity of weaned piglets.Dried AS herb was decocted in boiling distilled water(200 g/L)for 2 h.The decoction products were filtered and lypholized.The yield of extract was about 25%(w/w),with total polysaccherides,flavone and organic acids 2.94,0.19 and 1.04%,respectively.Ninety-six Duroc×Landrace×Yorkshire piglets weaned at 21 days of age(BW=5.64±0.48 kg)were randomly assigned to one of 3 treatments with 4 replicates each and fed a basal diet(BD),BD+ASE(1g/kg),and BD+antibiotics(10% bacitmein zinc 400 nag+15%carbadox 300 mg)for 14 days.Blood samples were collected on d7 and 14.ADG in the ASE group(216.80 g/d)and antibiotics group(231.38 g/d)were 16.81(P＞0.05)and 24.66%(P＜0.05)higher than the control group,and gain-feed ratio was improved by 18.18 and 21.39%(P＜0.05),respectively.However,there was no difference in growth performance between the ASE and antibiotic group(P＞0.05).Compared to the control group,both ASE and antibiotics increased Cu-Zn superoxide dismutase(SOD)activity on d 7 and 14(P＜0.05).SoD activity decreased from 147.96±15.88 to 125.68±14.26 in the antibiotics group(P＜0.05),while increased in the ASE group(from 132.37±7.64 to 135.44±12.68).Malondialdehyde in the ASE group significantly decreased by 20.00 and 30.46%(P＜0.05)on d 7 and 14,respectively.Glutathione also had an increasing trend in the ASE group on d 7 and 14(P=0.235 and 0.211,respectively).In addition,ASE influenced serum IgG on d 14(P＜0.05).These findings indicted that ASE could alleviate weaning stress by improving antioxidant enzymatic system effectively in piglets.