CAJ | 학술논문

[目的]对宁夏六盘山区黄牛杂交改良群体生长激素受体(GHR)基因的多态性进行分析,为宁夏黄牛杂交改良选育提供理论参考.[方法]采用聚合酶链式反应-限制性片段长度多态性方法(PCR-RFLP),对该群体70个个体的GHR基因多态性进行检测.[结果]扩增出338bp的目的片段,PCR产物被限制性内切酶Alu I消化后表现出多态性,其中AA基因型频率为75.71%;BB基因型频率为24.29%.[结论]共检测到AA和BB 2种基因型,六盘山区黄牛杂交改良群体GHR基因具有多态性.
[목적]대저하륙반산구황우잡교개량군체생장격소수체(GHR)기인적다태성진행분석,위저하황우잡교개량선육제공이론삼고.[방법]채용취합매련식반응-한제성편단장도다태성방법(PCR-RFLP),대해군체70개개체적GHR기인다태성진행검측.[결과]확증출338bp적목적편단,PCR산물피한제성내절매Alu I소화후표현출다태성,기중AA기인형빈솔위75.71%;BB기인형빈솔위24.29%.[결론]공검측도AA화BB 2충기인형,륙반산구황우잡교개량군체GHR기인구유다태성.
[Objective] The paper aimed to analyze the polymorphism of the growth hormone receptor (GHR) in improved hybrid yellow cattle group from Liupan Mountain area in Ningxia Autonomous Region, so as to provide technological basis for hybrid improvement. [Method] Polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) technology was carried out to examine polymorphisms of GHR gene of 70individuals. [Result] The target fragment of 338 bp was amplified. The PCR product digested by restriction enzyme Alu I showed polymorphisms.The frequencies of the two genotypes ( AA, BB) were 75.71% (53 individuals) and 24.29% ( 17 individuals ), respectively. [Conclusion] Two genotypes of GHR gene were detected in improved hybrid yellow cattle groups from Liupan Mountain area in Ningxia.