CAJ | 학술논문

介绍一种不经热浴快速从双孢蘑菇子实体中提取DNA的新方法,并与传统的CTAB和SDS法相比较,结果显示:该方法得到的DNA完整性好,时间短,步骤少,有毒试剂用量少,在提取DNA的质量、得率、时间及完整性等方面都优于传统的CTAB和SDS法,为食药用菌子实体DNA的提取参考.
개소일충불경열욕쾌속종쌍포마고자실체중제취DNA적신방법,병여전통적CTAB화SDS법상비교,결과현시:해방법득도적DNA완정성호,시간단,보취소,유독시제용량소,재제취DNA적질량、득솔、시간급완정성등방면도우우전통적CTAB화SDS법,위식약용균자실체DNA적제취삼고.
A new,simple and rapid method for obtaining DNA from Agaricus bisporus fruit bodies is described and compared with the widely used CTAB and SDS methods in terms of yield,DNA quality and integrity,and duration of extraction time.Total DNA quality was estimated by restriction endonuclease digestion and by ITS,ISSR and RAPD analyses using standard protocols.Our data demonstrated that the new method rcnders improved yields of total DNA of high quality and integrity,and is less time-consuming than existing procedures.