CAJ | 학술논문

目的观察感染尖吻蝮蛇舌形虫小鼠体内特异性抗体和循环抗原动态变化.方法从感染尖吻蝮蛇舌形虫的五步蛇体内收集成虫,分离尖吻蝮蛇舌形虫的虫卵感染小鼠,从感染后1 w、2 w、3～22 w收集小鼠血清,分别用ELISA法和dot-ELISA法观察不同时间小鼠体内尖吻蝮蛇舌形虫特异性抗体和循环抗原的动态变化.结果感染尖吻蝮蛇舌形虫的小鼠,其体内特异性抗体从第8 w开始上升,12w达到高峰,第16w开始下降并一直维持同一水平.同时,对小鼠产生的特异性抗体进行分型,其最早出现为IgM,16w以后被IgG1所替代.感染尖吻蝮蛇舌形虫的小鼠血清用dot-ELISA法检测其循环抗原出现的时间为第1 w,到第3 w时循环抗原检出稀释度在1:8～1:128之间,到第8 w,最高稀释度可达到1:256,并一直维持,第11w以后逐渐下降.结论 1.小鼠感染尖吻蝮蛇舌形虫虫卵后特异性抗体(Ab)在感染第8 w开始上升,抗体最高滴度维持时间为第12～15 w.2.感染尖吻蝮蛇舌形虫虫卵的小鼠其血清中产生的特异性抗体最早出现为IgM,以后被IgG1所替代.3.小鼠在感染尖吻蝮蛇舌形虫虫卵后的1 w就可在其血清中检测到循环抗原(CAg),预测这段时间检测循环抗原具有早期诊断参考价值.
목적 관찰감염첨문복사설형충소서체내특이성항체화순배항원동태변화.방법 종감염첨문복사설형충적오보사체내수집성충,분리첨문복사설형충적충란감염소서,종감염후1 w、2 w、3～22 w수집소서혈청,분별용ELISA법화dot-ELISA법관찰불동시간소서체내첨문복사설형충특이성항체화순배항원적동태변화.결과 감염첨문복사설형충적소서,기체내특이성항체종제8 w개시상승,12w체도고봉,제16w개시하강병일직유지동일수평.동시,대소서산생적특이성항체진행분형,기최조출현위IgM,16w이후피IgG1소체대.감염첨문복사설형충적소서혈청용dot-ELISA법검측기순배항원출현적시간위제1 w,도제3 w시순배항원검출희석도재1:8～1:128지간,도제8 w,최고희석도가체도1:256,병일직유지,제11w이후축점하강.결론 1.소서감염첨문복사설형충충란후특이성항체(Ab)재감염제8 w개시상승,항체최고적도유지시간위제12～15 w.2.감염첨문복사설형충충란적소서기혈청중산생적특이성항체최조출현위IgM,이후피IgG1소체대.3.소서재감염첨문복사설형충충란후적1 w취가재기혈청중검측도순배항원(CAg),예측저단시간검측순배항원구유조기진단삼고개치.
In this study,adults of Armillifer agkistrodontis (A.agkistrodontis) were collected from Agkistrodon acutus,and then the eggs were separated to feed mice.In the next step,when the infection model was established,blood serum of infected mice were collected after 1,2 and 3 weeks,respectively.Furthermore,ELISA and dot- ELISA were used to detect the dynamic change of specific antibodies and circulating antigens respectively.The specific antibodies increased from 8th week,reached the top at 12th week,decreased from 16th week,and then maintain at the same level constantly.Meanwhile,the specific antibodies were typed.It is evident that IgM antibody appeared first.However,it was substitute by IgG1 after 16 weeks.Moreover,the circulating antigens have been detected in the 1st week by dot-ELISA.Then,the dilution between 1:8 to 1:128were founded in 3rd week.The highest dilution with 1:256 appeared at 8th week,maintained before 11th week and then decreased gradually,which might provide a significant clinical implication for early diagnosis of circulating antigens.