CAJ | 학술논문

目的探讨以血管内皮生长因子受体2 (VEGFR2)为靶点的靶向超声造影评价裸鼠肾癌新生血管的应用价值.方法建立人肾透明细胞癌皮下移植瘤裸鼠模型,制备携VEGFR2抗体的靶向造影剂(MBV),每只裸鼠分别随机注射普通造影剂(MBC)和MBV并行超声造影检查,比较两种造影剂的视频强度,并对肿瘤组织行VEGFR2免疫组化检测.结果超声造影示MBV组视频强度(6.50±1.43)Db,MBC组视频强度(2.59±0.99)Db,两者差异有统计学意义(P<0.01),且MBV组持续强化时间较MBC组长,免疫组化结果证实肿瘤血管内皮VEGFR2高表达.结论以VEGFR2为靶点的靶向造影剂可特异性增强肾癌显影,对评估肿瘤新生血管有重要价值.
목적 탐토이혈관내피생장인자수체2 (VEGFR2)위파점적파향초성조영평개라서신암신생혈관적응용개치.방법 건립인신투명세포암피하이식류라서모형,제비휴VEGFR2항체적파향조영제(MBV),매지라서분별수궤주사보통조영제(MBC)화MBV병행초성조영검사,비교량충조영제적시빈강도,병대종류조직행VEGFR2면역조화검측.결과 초성조영시MBV조시빈강도(6.50±1.43)Db,MBC조시빈강도(2.59±0.99)Db,량자차이유통계학의의(P<0.01),차MBV조지속강화시간교MBC조장,면역조화결과증실종류혈관내피VEGFR2고표체.결론 이VEGFR2위파점적파향조영제가특이성증강신암현영,대평고종류신생혈관유중요개치.
Objective To investigate the value of contrast enhanced ultrasound(CEUS) with microbubbles targeted to vascular endothelial growth factor receptor type 2 (VEGFR2) for imaging tumor angiogenesis in murine tumor models.Methods Established human renal cell carcinomas(RCC) subcutaneous xenograft tumor model in nude mice,microbubbles targeted to VEGFR2(MBV) was prepared,control microbubbles(MBC) and MBV were injected respectively in each mouse,the intensity of each microbubble was compared,the expressions of VEGFR2 in tumors were tested by immunohistochemistry.Results CEUS imaging showed the intensity of MBV and MBC was (6.50±1.43)dB,(2.59±0.99)dB,respectively.There was significantly higher intensity when using MBV compared with MBC (P<0.01).The time to wash out was longer in MBV contrast group compared with MBC group.Immunohistochemistry showed VEGFR2 was highly expressed in novel vessel walls.Conclusions Contrast microbubbles targeted to VEGFR2 can specially enhance the images of RCC and has tremendous significance in the assessment of angiogenesis.