中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2012年
4期
226-230
,共5页
马书敏%杜文军%郑昭敏%甄军晖%陈士俊
馬書敏%杜文軍%鄭昭敏%甄軍暉%陳士俊
마서민%두문군%정소민%견군휘%진사준
肝炎病毒,乙型%肾小球肾炎%半胱氨酸天冬氨酸蛋白酶1%白细胞介素1β
肝炎病毒,乙型%腎小毬腎炎%半胱氨痠天鼕氨痠蛋白酶1%白細胞介素1β
간염병독,을형%신소구신염%반광안산천동안산단백매1%백세포개소1β
Hepatitis B virus%Glomerulonephritis%Caspase 1%Interleukin-1β
目的 初步探讨黑素瘤缺乏因子2(AIM2)激活与表达在HBV相关性肾小球肾炎(HBV-GN)发病机制中的作用.方法 54例HBV-GN患者为研究组,25例慢性肾小球肾炎患者为阴性对照组,6例慢性乙型肝炎患者为阳性对照组.免疫组织化学法分别测定研究组、阴性对照组患者肾组织及阳性对照组患者肝组织中AIM2、Caspase-1、IL-1β的表达.组间比较采用卡方检验,相关性分析采用Spearman相关分析.结果 54例HBV-GN患者肾组织中AIM2的阳性表达率为81.4%,25例慢性肾小球肾炎组为4.0%,两组间差异有统计学意义(x2=38.746,P<0.01).AIM2在6例慢性乙型肝炎患者肝组织中均有表达.HBV-GN组AIM2的表达强度与Caspase-1呈正相关(rs=0.444,P<0.01),IL-1β的表达强度与Caspase-1也呈正相关(rs=0.515,P<0.01).HBV-GN患者中血HBV DNA≥1×105拷贝/mL者AIM2的表达强度高于HBV DNA<1×105拷贝/mL者(x2=6.097,P<0.05).结论 AIM2的激活与HBV-GN有关,可能通过Caspase-1激活,释放炎性因子IL-1β.
目的 初步探討黑素瘤缺乏因子2(AIM2)激活與錶達在HBV相關性腎小毬腎炎(HBV-GN)髮病機製中的作用.方法 54例HBV-GN患者為研究組,25例慢性腎小毬腎炎患者為陰性對照組,6例慢性乙型肝炎患者為暘性對照組.免疫組織化學法分彆測定研究組、陰性對照組患者腎組織及暘性對照組患者肝組織中AIM2、Caspase-1、IL-1β的錶達.組間比較採用卡方檢驗,相關性分析採用Spearman相關分析.結果 54例HBV-GN患者腎組織中AIM2的暘性錶達率為81.4%,25例慢性腎小毬腎炎組為4.0%,兩組間差異有統計學意義(x2=38.746,P<0.01).AIM2在6例慢性乙型肝炎患者肝組織中均有錶達.HBV-GN組AIM2的錶達彊度與Caspase-1呈正相關(rs=0.444,P<0.01),IL-1β的錶達彊度與Caspase-1也呈正相關(rs=0.515,P<0.01).HBV-GN患者中血HBV DNA≥1×105拷貝/mL者AIM2的錶達彊度高于HBV DNA<1×105拷貝/mL者(x2=6.097,P<0.05).結論 AIM2的激活與HBV-GN有關,可能通過Caspase-1激活,釋放炎性因子IL-1β.
목적 초보탐토흑소류결핍인자2(AIM2)격활여표체재HBV상관성신소구신염(HBV-GN)발병궤제중적작용.방법 54례HBV-GN환자위연구조,25례만성신소구신염환자위음성대조조,6례만성을형간염환자위양성대조조.면역조직화학법분별측정연구조、음성대조조환자신조직급양성대조조환자간조직중AIM2、Caspase-1、IL-1β적표체.조간비교채용잡방검험,상관성분석채용Spearman상관분석.결과 54례HBV-GN환자신조직중AIM2적양성표체솔위81.4%,25례만성신소구신염조위4.0%,량조간차이유통계학의의(x2=38.746,P<0.01).AIM2재6례만성을형간염환자간조직중균유표체.HBV-GN조AIM2적표체강도여Caspase-1정정상관(rs=0.444,P<0.01),IL-1β적표체강도여Caspase-1야정정상관(rs=0.515,P<0.01).HBV-GN환자중혈HBV DNA≥1×105고패/mL자AIM2적표체강도고우HBV DNA<1×105고패/mL자(x2=6.097,P<0.05).결론 AIM2적격활여HBV-GN유관,가능통과Caspase-1격활,석방염성인자IL-1β.
Objective To explore the role of activation and expression of absent in melanoma 2 (AIM2) in pathogenesis of hepatitis B virus (HBV) associated glomerulonephritis (HBV-GN).Methods Fifty-four patients with HBV-GN were recruited into the investigational group,and 25 patients with chronic glomerulonephritis (CGN) and 6 patients with chronic hepatitis B (CHB) were included as negative control group and positive control group,respectively.Immunohistochemistry was used to detect AIM2,Caspase-1 and interleukin (IL)-1β expressions in both kidney tissues from the investigational group and negative control group and liver tissues from positive control group.The differences between groups were compared by chi-square test,and the correlation analysis was conducted by Spearman correlation test.Results The positive expression rates of AIM2 in 54 patients of HBV-GN group and 25 of CGN group were 81.4 % and 4.0%,respectively (x2 =38.746,P< 0.01).AIM2 was found to be expressed in all 6 CHB patients.In HBV-GN group,the expression of AIM2 was positively correlated with Caspase-1 (rs =0.444,P<0.01),and the expression of IL-1β was also positively correlated with Caspase-1 (rs =0.515,P<0.01 ).In HBV-GN group,the expression of AIM2 in patients with HBV DNA≥1 ×105 copy/mL was significantly higher than that in those with HBV DNA<1 × 105 copy/mL (x2 =6.097,P<0.05).Conclusion The activation of AIM2 may play a role in the pathogenesis of HBV-GN through Caspase-1 activation and inflammatory factor IL-1β release.