CAJ | 학술논문

目的研究α-细辛醚的体内抗运动性疲劳作用和对骨骼肌及脑组织自由基代谢的影响.方法将50只大鼠随机分成正常对照组,α-细辛醚组,运动组,运动+α-细辛醚组和运动+咖啡因组,每组10只.观察α-细辛醚对运动大鼠疲劳相关症状、跑台力竭时间和脑组织及骨骼肌组织SOD和MDA的影响.结果实验第三周末运动组大鼠活动减少,毛发枯萎,无光泽,食欲减少.运动+α-细辛醚组和运动+咖啡因组大鼠与运动组比较,上述疲劳症状明显减轻.运动组大鼠脑组织和骨骼肌组织SOD活力分别为(26.63±7.85)NU/mg和(30.97±6.41)NU/mg均高于正常对照组(20.84±4.53)NU/mg和(24.21±3.74)NU/mg以及α-细辛醚组(21.12±3.38)NU/mg和(23.53±5.26)NU/mg;低于运动+α-细辛醚组(32.64±8.35)NU/mg和(37.64±9.75)NU/mg(P＜0.05);与运动+咖啡因组(27.47±5.22)NU/mg和(32.22±6.89)NU/mg比较,差异无显著性(P＞0.05).运动组大鼠脑组织和骨骼肌组织MDA含量分别为:(7.91±1.90)nmol/ml和(9.61±2.11)nmol/ml均高于正常对照组(2.73±1.13)nmol/ml和(3.89±1.35)nmol/ml、α-细辛醚组(2.71±1.03)nmol/ml和(3.77±1.45)nmol/ml和运动+α-细辛醚组(5.23±2.01)nmol/ml和(6.17±1.57)nmol/ml(P＜0.05);与运动+咖啡因组(8.05±2.29)nmol/ml和(8.01±1.45)nmol/ml比较,差异无显著性(P＞0.05).运动+α-细辛醚组大鼠脑组织和骨骼肌组织SOD活力高于其余各组(P＜0.05);而MDA含量低于其余各组,(P＜0.05).结论 α-细辛醚具有较明显的抗运动性疲劳作用,其机理与α-细辛醚纠正疲劳机体自由基代谢失衡有关.
목적 연구α-세신미적체내항운동성피노작용화대골격기급뇌조직자유기대사적영향.방법 장50지대서수궤분성정상대조조,α-세신미조,운동조,운동+α-세신미조화운동+가배인조,매조10지.관찰α-세신미대운동대서피로상관증상、포태력갈시간화뇌조직급골격기조직SOD화MDA적영향.결과 실험제삼주말운동조대서활동감소,모발고위,무광택,식욕감소.운동+α-세신미조화운동+가배인조대서여운동조비교,상술피로증상명현감경.운동조대서뇌조직화골격기조직SOD활력분별위(26.63±7.85)NU/mg화(30.97±6.41)NU/mg균고우정상대조조(20.84±4.53)NU/mg화(24.21±3.74)NU/mg이급α-세신미조(21.12±3.38)NU/mg화(23.53±5.26)NU/mg;저우운동+α-세신미조(32.64±8.35)NU/mg화(37.64±9.75)NU/mg(P＜0.05);여운동+가배인조(27.47±5.22)NU/mg화(32.22±6.89)NU/mg비교,차이무현저성(P＞0.05).운동조대서뇌조직화골격기조직MDA함량분별위:(7.91±1.90)nmol/ml화(9.61±2.11)nmol/ml균고우정상대조조(2.73±1.13)nmol/ml화(3.89±1.35)nmol/ml、α-세신미조(2.71±1.03)nmol/ml화(3.77±1.45)nmol/ml화운동+α-세신미조(5.23±2.01)nmol/ml화(6.17±1.57)nmol/ml(P＜0.05);여운동+가배인조(8.05±2.29)nmol/ml화(8.01±1.45)nmol/ml비교,차이무현저성(P＞0.05).운동+α-세신미조대서뇌조직화골격기조직SOD활력고우기여각조(P＜0.05);이MDA함량저우기여각조,(P＜0.05).결론 α-세신미구유교명현적항운동성피노작용,기궤리여α-세신미규정피로궤체자유기대사실형유관.
Objective Effects of cis-asarone from Acorus Tatarinowii Schott were investigated on anti excise-induced fatigue to free radicals metabolism in skeletal muscle and brain tissue of overtrained rats. Methods Fifty rats were divided into 5 groups: the control group, the cis-asarone group, the exercise group, the exercise plus cis-asarone group, the exercise plus caffeine group with ten rats in each group to study the effects of cis-asarone on fatigue-related symptoms, all-out time, superoxide dismutase (SOD) activity and malonyldialdehyde (MDA) levels of tissues including brain tissue and skeletal muscle of overtrained rats. Results After 3 weeks of experiment, the rats in the exercise group showed reduced activity, thinned fur, decreased appetite, while those symptoms significantly lessened in the rats of the exercise plus cis-asarone group and the exercise plus caffeine group. The all-out time of rats in the exercise plus cis-asarone group(82.53±11.40) min were significantly longer than those of the cis-asarone group (70.26±10.78 min), the exercise group(66.16±15.45 min)(P＜0.05),but they were significantly shorter than those of the exercise plus caffeine group (103.01±8.21 min) (P＜0.01).The SOD activity of the brain tissues (26.63±7.85) NU/mg and skeletal muscle (30.97±6.41) NU/mg in rats in the exercise group was significantly higher than that in the control group [(20.84±4.53)NU/mg and (24.21±3.74)NU/mg], the cis-asarone group [(32.64±8.35) NU/mg and (37.64±9.75) NU/mg], lower than the exercise plus cis-asarone group [(32.64±8.35) NU/mg and (37.64±9.75) NU/mgl(P＜0.05).But there is no significant difference between the exercise group and the exercise plus caffeine group [(27.47±5.22) NU/mg and (32.22±6.89) NU/mg](P＜0.05). The levels of MDA of the brain tissues (7.91±1.90) nmol/ml] and skeletal muscle [(9.61±2.11) nmol/ml]in rats in the exercise group was significantly higher than those in the control group [(2.73±1.13) nmol/ml and (3.89±1.35) nmol/mll, the cis-asarone group [(2.71±1.03) nmol/ml and (3.77±1.45) nmol/ml]and the exercise plus cis-asarone group [(5.23±2.01) nmol/ml and (6.17±1.57) nmol/mll (P＜0.05). But there is no significant difference between the exercise group and the exercise plus caffeine group [(8.05±2.29) nmol/ml and (8.01±1.45) nmol/ml](P＞0.05). The SOD activity of the brain tissues and skeletal muscle in rats in the exercise plus cis-asarone group was significantly higher than those of the other groups (P＜0.05), while the levels of MDA were opposite (P＜0.05). Conclusion Cis-asarone was found to have significant effects on retarding fatigue, improving performance through high SOD activity,facilitating free radicals removal, and thus protecting the tissues from free radical damage.