中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2012年
5期
325-328
,共4页
杨斌%周细玉%郑和平%薛耀华
楊斌%週細玉%鄭和平%薛耀華
양빈%주세옥%정화평%설요화
衣原体,沙眼%小鼠%模型,动物
衣原體,沙眼%小鼠%模型,動物
의원체,사안%소서%모형,동물
Chlamydia trachomatis%Mice%Models,animal
目的 建立不同血清型沙眼衣原体小鼠生殖道感染模型,为研究型别与毒力的关系奠定基础.方法 将6周龄BALB/c雌性小鼠分为4组,实验组经孕酮处理后接种4.0× 107沙眼衣原体,孕酮对照组接种McCoy细胞培养液,无孕酮对照组直接接种4.0×107沙眼衣原体,以及单纯小鼠的空白对照.分别于接种后4d起观察小鼠外阴变化,阴道分泌物细胞培养、直接免疫荧光法和实时荧光PCR检测沙眼衣原体.结果 实验组接种沙眼衣原体E、F、J和K型,4d后小鼠生殖道表现出轻微的感染迹象,7d后阴道分泌物培养、免疫荧光和PCR检测均检出沙眼衣原体,而对照组为阴性.感染持续时间以K型最长(21 d,1/11只阳性),其次为J型(17 d,1/11只阳性)、F型(14 d,5/11只阳性)和E型(14d,2/11只阳性).结论 适龄小鼠经孕酮处理后,接种一定量的沙眼衣原体,可建立不同血清型沙眼衣原体感染模型.
目的 建立不同血清型沙眼衣原體小鼠生殖道感染模型,為研究型彆與毒力的關繫奠定基礎.方法 將6週齡BALB/c雌性小鼠分為4組,實驗組經孕酮處理後接種4.0× 107沙眼衣原體,孕酮對照組接種McCoy細胞培養液,無孕酮對照組直接接種4.0×107沙眼衣原體,以及單純小鼠的空白對照.分彆于接種後4d起觀察小鼠外陰變化,陰道分泌物細胞培養、直接免疫熒光法和實時熒光PCR檢測沙眼衣原體.結果 實驗組接種沙眼衣原體E、F、J和K型,4d後小鼠生殖道錶現齣輕微的感染跡象,7d後陰道分泌物培養、免疫熒光和PCR檢測均檢齣沙眼衣原體,而對照組為陰性.感染持續時間以K型最長(21 d,1/11隻暘性),其次為J型(17 d,1/11隻暘性)、F型(14 d,5/11隻暘性)和E型(14d,2/11隻暘性).結論 適齡小鼠經孕酮處理後,接種一定量的沙眼衣原體,可建立不同血清型沙眼衣原體感染模型.
목적 건립불동혈청형사안의원체소서생식도감염모형,위연구형별여독력적관계전정기출.방법 장6주령BALB/c자성소서분위4조,실험조경잉동처리후접충4.0× 107사안의원체,잉동대조조접충McCoy세포배양액,무잉동대조조직접접충4.0×107사안의원체,이급단순소서적공백대조.분별우접충후4d기관찰소서외음변화,음도분비물세포배양、직접면역형광법화실시형광PCR검측사안의원체.결과 실험조접충사안의원체E、F、J화K형,4d후소서생식도표현출경미적감염적상,7d후음도분비물배양、면역형광화PCR검측균검출사안의원체,이대조조위음성.감염지속시간이K형최장(21 d,1/11지양성),기차위J형(17 d,1/11지양성)、F형(14 d,5/11지양성)화E형(14d,2/11지양성).결론 괄령소서경잉동처리후,접충일정량적사안의원체,가건립불동혈청형사안의원체감염모형.
Objective To establish a mouse model for genital tract infection with different serovars of Chlamydia trachomatis (Ct),and to provide a basis for understanding the relationship between Ct serovars and virulence.Methods Six-week-old female BALB/c mice were divided into 4 groups:study group (pretreated with progesterone and inoculated with 4.0 × 107 inclusion-forming units (IFU) of Ct serovar E,F,J and K,respectively),progesterone-control group (pretreated with progesterone and inoculated with culture medium of McCoy cells),non progesterone-control group (inoculated with 4.0 × 107 IFU of Ct serovar F),blank control group receiving no treatment.At 4 and 7 days after the inoculation,the appearance of vulva was observed,vaginal swab specimens were obtained and subjected to cell culture,direct immunofluorescence assay and real-time fluorescence-based PCR for the detection of Ct.Results A slight inflammation of the genital tract was observed in the mice 4 days after the inoculation with Ct serovar E,F,J or K in the study group.Cell culture,direct immunofluorescence assay and real-time fluorescence-based PCR all confirmed that Ct was present in vaginal specimens from the study group,but absent from the control groups.The longest duration of infection was 21 days in 1 out of 11 mice inoculated with Ct serovar K,followed by 17 days in 1 out of 11 mice inoculated with Ct serovar J,14 days in 5 out of 11 mice with Ct serovar F and in 2 out of 11 mice with Ct serovar K.Conclusion The model for genital tract infection with different serovars of Ct can be established in mice at a right age bypretreatment with progesterone and inoculation with a certain amount of Ct.
