中华消化杂志
中華消化雜誌
중화소화잡지
Chinese Journal of Digestion
2012年
5期
330-333
,共4页
食管肿瘤%癌,鳞状细胞%血管内皮生长因子类%血管紧张素转化酶抑制药%培哚普利%苯丙氮卓类%小鼠,裸
食管腫瘤%癌,鱗狀細胞%血管內皮生長因子類%血管緊張素轉化酶抑製藥%培哚普利%苯丙氮卓類%小鼠,裸
식관종류%암,린상세포%혈관내피생장인자류%혈관긴장소전화매억제약%배타보리%분병담탁류%소서,라
Esophageal neoplasms%Carcinoma,squamous cell%Vascular endothelial growth factors%Angiotensin converting enzyme inhibitors%Perindopril%Benzazepines%mice,nude
目的 探索血管紧张素Ⅰ转化酶抑制剂(ACEI)在食管鳞癌细胞株中是否存在抑瘤作用.方法 应用RT-PCR法在食管鳞癌细胞株KYSE30、TE-1、EC109、EC9706中筛选出血管内皮生长因子(VEGF) mRNA表达水平最高者,并将其用于接种和建立裸鼠移植瘤模型.成瘤后将裸鼠均分为对照组、培哚普利组、苯那普利组(n=6),分别予0.9％ NaCl溶液、培哚普利(4 mg/kg)、苯那普利(6 mg/kg)干预.测定各组裸鼠移植瘤的体积,计算抑瘤率.免疫组织化学法检测裸鼠移植瘤瘤体组织内CD31的表达,并计算肿瘤微血管密度(MVD).结果 EC9706细胞株中VEGFmRNA表达水平最高.在第2和第3周时,各组移植瘤体积无明显差异.在第4和第5周时,培哚普利组移植瘤体积与对照组比较无明显差异,抑瘤率分别为24.6％和21.1％,苯那普利组和对照组比较差异均有统计学意义(t=-2.450和-3.120,P=0.035和0.008),抑瘤率分别为33.1％和45.4％.经培哚普利和苯那普利干预后裸鼠移植瘤组织内CD31表达水平有所降低.苯那普利组MVD明显低于对照组(10.98±1.18比13.98±1.76,t=-3.732,P=0.002),培哚普利组(12.41±1.15)则与对照组差异无统计学意义(t=-2.053,P=0.07).结论 苯那普利(6 mg/kg)能明显抑制EC9706裸鼠移植瘤生长,抑制肿瘤新生血管形成可能是其抑瘤机制之一.培哚普利(4 mg/kg)可能存在类似作用.
目的 探索血管緊張素Ⅰ轉化酶抑製劑(ACEI)在食管鱗癌細胞株中是否存在抑瘤作用.方法 應用RT-PCR法在食管鱗癌細胞株KYSE30、TE-1、EC109、EC9706中篩選齣血管內皮生長因子(VEGF) mRNA錶達水平最高者,併將其用于接種和建立裸鼠移植瘤模型.成瘤後將裸鼠均分為對照組、培哚普利組、苯那普利組(n=6),分彆予0.9％ NaCl溶液、培哚普利(4 mg/kg)、苯那普利(6 mg/kg)榦預.測定各組裸鼠移植瘤的體積,計算抑瘤率.免疫組織化學法檢測裸鼠移植瘤瘤體組織內CD31的錶達,併計算腫瘤微血管密度(MVD).結果 EC9706細胞株中VEGFmRNA錶達水平最高.在第2和第3週時,各組移植瘤體積無明顯差異.在第4和第5週時,培哚普利組移植瘤體積與對照組比較無明顯差異,抑瘤率分彆為24.6％和21.1％,苯那普利組和對照組比較差異均有統計學意義(t=-2.450和-3.120,P=0.035和0.008),抑瘤率分彆為33.1％和45.4％.經培哚普利和苯那普利榦預後裸鼠移植瘤組織內CD31錶達水平有所降低.苯那普利組MVD明顯低于對照組(10.98±1.18比13.98±1.76,t=-3.732,P=0.002),培哚普利組(12.41±1.15)則與對照組差異無統計學意義(t=-2.053,P=0.07).結論 苯那普利(6 mg/kg)能明顯抑製EC9706裸鼠移植瘤生長,抑製腫瘤新生血管形成可能是其抑瘤機製之一.培哚普利(4 mg/kg)可能存在類似作用.
목적 탐색혈관긴장소Ⅰ전화매억제제(ACEI)재식관린암세포주중시부존재억류작용.방법 응용RT-PCR법재식관린암세포주KYSE30、TE-1、EC109、EC9706중사선출혈관내피생장인자(VEGF) mRNA표체수평최고자,병장기용우접충화건립라서이식류모형.성류후장라서균분위대조조、배타보리조、분나보리조(n=6),분별여0.9％ NaCl용액、배타보리(4 mg/kg)、분나보리(6 mg/kg)간예.측정각조라서이식류적체적,계산억류솔.면역조직화학법검측라서이식류류체조직내CD31적표체,병계산종류미혈관밀도(MVD).결과 EC9706세포주중VEGFmRNA표체수평최고.재제2화제3주시,각조이식류체적무명현차이.재제4화제5주시,배타보리조이식류체적여대조조비교무명현차이,억류솔분별위24.6％화21.1％,분나보리조화대조조비교차이균유통계학의의(t=-2.450화-3.120,P=0.035화0.008),억류솔분별위33.1％화45.4％.경배타보리화분나보리간예후라서이식류조직내CD31표체수평유소강저.분나보리조MVD명현저우대조조(10.98±1.18비13.98±1.76,t=-3.732,P=0.002),배타보리조(12.41±1.15)칙여대조조차이무통계학의의(t=-2.053,P=0.07).결론 분나보리(6 mg/kg)능명현억제EC9706라서이식류생장,억제종류신생혈관형성가능시기억류궤제지일.배타보리(4 mg/kg)가능존재유사작용.
Objective To investigate whether there was inhibitory effect of angiotensin-Ⅰ converting enzyme inhibitors (ACEI) on esophageal carcinoma cell line.Methods The highest expression of vascular endothelial growth factor (VEGF) at mRNA level was screened in esophageal squamous cell carcinoma cell lines KYSE30,TE-1,EC109 and EC9706 by reverse transcriptionpolymerase chain reaction (RT-PCR) and which was transplanted and established nude mice xenografts model.After tumor formed,the nude mice were evenly divided into control group,perindopril group and benazepril group (n=6) of which 0.9％ saline,4 mg/kg perindopril and 6 mg/kg benazepril was given respectively to nude mice in each group.The tumor size and the tumor inhibitory rate were measured.The expression of CD31 in xenograft tumor was tested by immunohistochemistry and the microvessel density (MVD) was assessed.Results The highest expression of VEGF at mRNA level was in EC9706 cell line.At second and third week,there was no significant difference in xenograft tumor size between each group. At fourth and fifth week,there was no significant difference in xenograft tumor size between perindopril group and control group,and the tumor inhibitory rate was 24.6 ％ and 21.1 ％.There was significant difference between benazepril group and control group (t=-2.450 and -3.120,P=0.035 and 0.008),and the tumor inhibitory rate was 33.1％ and 45.4％.After treated with perindopril and benazepril,the expression of CD31 in xenograft tumor of nude mice decreased.The MVD of benazepril group was significantly lower than that of control group (10.98±1.18 vs 13.98 ± 1.76； t =-3.732,P =0.002),and there was no significant difference between perindopril group (12.41±1.15) and control group (t=-2.053,P=0.07).Conclusions Benazepril (6 mg/kg) could significantly inhibit the growth of EC9706 cell line formed xenograft tumor of nude mice.One of the possible tumor inhibitory mechanisms was inhibiting new vessel forming in tumor.Perindopril (4 mg/kg) may have the similar effect.
