CAJ | 학술논문

该文分别选用不同浓度的海螺酶；葡萄糖、蔗糖、甘露醇、山梨醇、氯化钠5种渗透剂；维生素C和甘露醇2种抗氧化剂；对条斑紫菜酶解单细胞的成活率进行了研究。实验结果表明：用浓度为10%的海螺酶酶解条斑紫菜，成活率最高；选用2mol/L葡萄糖作渗透剂，效果最好，成活率达 85.2%；加入抗氧化剂对细胞成活率没有影响。
해문분별선용불동농도적해라매；포도당、자당、감로순、산리순、록화납5충삼투제；유생소C화감로순2충항양화제；대조반자채매해단세포적성활솔진행료연구。실험결과표명：용농도위10%적해라매매해조반자채，성활솔최고；선용2mol/L포도당작삼투제，효과최호，성활솔체 85.2%；가입항양화제대세포성활솔몰유영향。
Effects of different enzymalysis conditions on the survival rate of single-cells of Porphyra yezoensis Ueda have been studied. The main results are: 1. The effectiveness of enzyme was positively correlated to enzyme concentration. When the enzyme concentration was 10% the result was the best. 2. Different penetrants showed different effects on cell survival rate. Among 5 kinds of penetrants, Glucose was the best. 3. Antioxidants had no effect on the cell survival rate.