CAJ | 학술논문

[目的]探讨有机添加物对大花蕙兰原球茎及其再生幼苗生长发育的影响.[方法]以大花蕙兰茎尖诱导的原球茎为材料,探讨不同有机添加物对原球茎及其分化幼苗生长发育的影响.[结果]结果表明,单独添加6种有机物时,0.2 g/L水解酪蛋白对原球茎增殖的效果最佳,增殖系数达13.10,其次分别为椰子汁、蛋白胨、土豆泥、香蕉泥、番茄汁;添加混合有机物时,50.0 ml/L香蕉泥与50.0 ml/L土豆泥混合物的增殖效果最佳,增殖系数达16.61;100.0 ml/L椰子汁对原球茎分化的效果最佳,原球茎分化芽数达11.37个/g,蛋白胨、香蕉泥,土豆泥、水解酪蛋白及番茄汁对原球茎分化具有抑制作用;添加50.0 ml/L香蕉泥、2.0 g/L蛋白胨与50.0 ml/L土豆泥混合物的培养基更适宜大花蕙兰幼苗生长发育.[结论]该研究结果为提高大花蕙兰种苗大规模工厂化生产效率提供技术参考.
[목적]탐토유궤첨가물대대화혜란원구경급기재생유묘생장발육적영향.[방법]이대화혜란경첨유도적원구경위재료,탐토불동유궤첨가물대원구경급기분화유묘생장발육적영향.[결과]결과표명,단독첨가6충유궤물시,0.2 g/L수해락단백대원구경증식적효과최가,증식계수체13.10,기차분별위야자즙、단백동、토두니、향초니、번가즙;첨가혼합유궤물시,50.0 ml/L향초니여50.0 ml/L토두니혼합물적증식효과최가,증식계수체16.61;100.0 ml/L야자즙대원구경분화적효과최가,원구경분화아수체11.37개/g,단백동、향초니,토두니、수해락단백급번가즙대원구경분화구유억제작용;첨가50.0 ml/L향초니、2.0 g/L단백동여50.0 ml/L토두니혼합물적배양기경괄의대화혜란유묘생장발육.[결론]해연구결과위제고대화혜란충묘대규모공엄화생산효솔제공기술삼고.
[Objective]The purpose of this research was to discuss the effect of organic additives on protocorm and seedling growth of Cymbidium.[Method]With the protocorm induced by Cymbidium stalk-tip as the materials, the effects of the organic additives on protocorm and seedling growth of Cymbidium were studied.[Result]The results indicated that under the alone using six kinds of organic additives, 0.2 g/L casein hydrolysate had the optimum effect on the protocorm proliferation, the multiplication coefficient was 13.10, followed by coconut milk, peptone, popato mesh, banana slurry and tomato juice, repecctively.When adding the mixed organics, the mixture of 50.0 ml/L banana slurry and 50.0 ml/L tomato juice had the optimum effect on the protocorm proliferation, the multiplication coefficient was 16.61.100.0 ml/L cocomut milk had the best effect on the protocorm defferentiation, the number of protocorm differentiation bud arrived 11.37个/g;peptone, popato mesh, banana slurry, tomato juice amd casein hydrolysate had obvious hibition on the protocorm defferentiation.The optium medium for growing and developing Cymbidium seedling was mixed organic additives of 50.0 ml/L banana slurry and 2.0 g/L peptone and 50.0 ml/L popato mesh.[Conclusion]This research will provide the technical reference for enhancing large-scale factory production efficiency of Cymbidium seedlings.