中南药学
中南藥學
중남약학
CENTRAL SOUTH PHARMACY
2009年
7期
540-544
,共5页
玄参%毛细管电泳指纹图谱%双定性相似度%双定量相似度%勾兑系数%破坏性试验%分解相似度
玄參%毛細管電泳指紋圖譜%雙定性相似度%雙定量相似度%勾兌繫數%破壞性試驗%分解相似度
현삼%모세관전영지문도보%쌍정성상사도%쌍정량상사도%구태계수%파배성시험%분해상사도
Raidix Scrophulariae%capillary electrophoresis fingerprint%dual qualitative similarities%dual quantitative similarities%blending coefficient%degradation test%decomposition similarity
目的 建立玄参药材的毛细管电泳指纹图谱(CEFP).方法 采用毛细管区带电泳法,以50 mmol·L-1硼砂为背景电解质,运行电压12.1 kV,检测波长280 nm,重力进样20 s(高度8.5 cm),以10个不同产地药材的电泳图谱建立了玄参CEFP.用"中药色谱指纹图谱超信息特征数字化评价系统3.0"软件获得相关指纹峰特征参数,用双定性相似度(SF与S′F)和双定量相似度(C与P)分别评价.对玄参CEFP进行了破坏性试验考察并考察了大峰和小峰分别缺失时4个相似度指标变化特征.结果 以哈巴俄苷峰为参照物峰,按共有峰出现率100%确定10个共有指纹峰,建立了玄参CEFP.采用DQDQS法评价出3批质量完全合格,4批含量明显偏低,3批含量明显偏高.破坏性试验结果表明玄参CEFP指纹在碱性条件下极易降解,其次在酸性条件和氧化作用下也发生降解.采用混批勾兑技术按含量相似度105%计算了两类含量高低不同样品的勾兑系数.结论 由SF与S′F和C与P构成的双定性双定量相似度(DQDQS)法能同时监测大峰和小峰的变动和缺失,能准确的解决色谱指纹图谱的宏观定性和定量评价的关键问题,所建立的CEFP为玄参药材质量控制提供了新方法.
目的 建立玄參藥材的毛細管電泳指紋圖譜(CEFP).方法 採用毛細管區帶電泳法,以50 mmol·L-1硼砂為揹景電解質,運行電壓12.1 kV,檢測波長280 nm,重力進樣20 s(高度8.5 cm),以10箇不同產地藥材的電泳圖譜建立瞭玄參CEFP.用"中藥色譜指紋圖譜超信息特徵數字化評價繫統3.0"軟件穫得相關指紋峰特徵參數,用雙定性相似度(SF與S′F)和雙定量相似度(C與P)分彆評價.對玄參CEFP進行瞭破壞性試驗攷察併攷察瞭大峰和小峰分彆缺失時4箇相似度指標變化特徵.結果 以哈巴俄苷峰為參照物峰,按共有峰齣現率100%確定10箇共有指紋峰,建立瞭玄參CEFP.採用DQDQS法評價齣3批質量完全閤格,4批含量明顯偏低,3批含量明顯偏高.破壞性試驗結果錶明玄參CEFP指紋在堿性條件下極易降解,其次在痠性條件和氧化作用下也髮生降解.採用混批勾兌技術按含量相似度105%計算瞭兩類含量高低不同樣品的勾兌繫數.結論 由SF與S′F和C與P構成的雙定性雙定量相似度(DQDQS)法能同時鑑測大峰和小峰的變動和缺失,能準確的解決色譜指紋圖譜的宏觀定性和定量評價的關鍵問題,所建立的CEFP為玄參藥材質量控製提供瞭新方法.
목적 건립현삼약재적모세관전영지문도보(CEFP).방법 채용모세관구대전영법,이50 mmol·L-1붕사위배경전해질,운행전압12.1 kV,검측파장280 nm,중력진양20 s(고도8.5 cm),이10개불동산지약재적전영도보건립료현삼CEFP.용"중약색보지문도보초신식특정수자화평개계통3.0"연건획득상관지문봉특정삼수,용쌍정성상사도(SF여S′F)화쌍정량상사도(C여P)분별평개.대현삼CEFP진행료파배성시험고찰병고찰료대봉화소봉분별결실시4개상사도지표변화특정.결과 이합파아감봉위삼조물봉,안공유봉출현솔100%학정10개공유지문봉,건립료현삼CEFP.채용DQDQS법평개출3비질량완전합격,4비함량명현편저,3비함량명현편고.파배성시험결과표명현삼CEFP지문재감성조건하겁역강해,기차재산성조건화양화작용하야발생강해.채용혼비구태기술안함량상사도105%계산료량류함량고저불동양품적구태계수.결론 유SF여S′F화C여P구성적쌍정성쌍정량상사도(DQDQS)법능동시감측대봉화소봉적변동화결실,능준학적해결색보지문도보적굉관정성화정량평개적관건문제,소건립적CEFP위현삼약재질량공제제공료신방법.
Objective To establish the capillary electrophoresis fingerprint (CEFP) of Raidix Scrophulariae. Methods CEFPs were obtained by capillary zone electrophoresis, in which the background electrolyte was 50 mmol·L-1 sodium borate. The voltage was 12.1 kV and the wavelength was set at 280 nm. The fingerprints were produced from electropherogram of 10 batches of samples. The 10 electropherograms were assessed by the software of the Digitized Evaluation System for Super-information Characteristics of the TCM Fingerprints, and also evaluated by dual qualitative similarities (SF and S′F) and dual quantitative similarities (C and P). The investigation of 5 degradation tests was made,and the variation of the 4 indexes was discussed when different large and small peaks were lost.Results Ten co-possessing peaks were selected as the characteristic fingerprint peaks of Raidix Scrophulariae when choosing harpagoside peak as the referential peak.The chemical component quantity of 3 batches of samples was completely qualified, while the dual quantitative similarities of 4 batches were very low and the contents of 3 batches were relatively high. The degradation test indicated that the fingerprints were much more unstable in basic condition and secondary in acidic condi-tion. Taking 105% as a norm,the blending coefficient between the high content crude drugs and the lower ones was successfully calculated.Conclusion SF can clearly reflect the chemical constituent distribution, and C can reflect the total contents of the sample, but both are seriously influenced by large peaks and can not reflect the missing of small peaks. Both S′F and P are of equal weight to the integrated area of all fingerprint peaks, and they can sensitively reflect the missing of small peaks. The dual qualitative similarities (SF and S′F) and dual quantitative similarities (C and P) are combined to accurately achieve the qualitative and quantitative assessment of quality of traditional Chinese medicine,for the changes or lacks of both big peaks and small peaks can be monitored simultaneously for fingerprints evaluation. It certainly can serve as a novel way to identify and control the quality of Raidix Scrophulariae.
