CAJ | 학술논문

通过建立高效率"双无"(无启动子、无polyA)打靶载体MSTN-GFP和MSTN-neo及新生绵羊和胎儿成肌细胞的培养和鉴定, 优化了培养条件, 改善了细胞状态, 为提高打靶效率及体细胞克隆提供了稳定的核移植供体;同时无启动子打靶载体的构建也有利于打靶后阳性细胞克隆的分子鉴定.
통과건립고효솔"쌍무"(무계동자、무polyA)타파재체MSTN-GFP화MSTN-neo급신생면양화태인성기세포적배양화감정, 우화료배양조건, 개선료세포상태, 위제고타파효솔급체세포극륭제공료은정적핵이식공체;동시무계동자타파재체적구건야유리우타파후양성세포극륭적분자감정.
We reported an investigation designed to knockout the MSTN gene by gene targeting in ovine myoblast cells. To increase the gene target efficiency and to supply nuleus transplantation donator for somatic cloning, two double traps (promoter-trap and polyA-trap) and targeting vectors MSTN-green fluorescent protein (GFP) and MSTN-neo were constructed and the foetal and neonatal ovine primary myoblast were cultured and identified. The cell cultural conditions were optimized. At the same time, the construct of no promoter vector was easy to molecular identification of positive cell clone after gene targetting.