CAJ | 학술논문

以浙江诸暨淡水文石珍珠为实验原料,提取文石珍珠中的两种基质蛋白--酸可溶蛋白(ASM)和水可溶有蛋白(WSM),并分别将其制备成一系列不同浓度的基质蛋白溶液.利用上述两种不同浓度的可溶性有机基质溶液采用扩散法开展了碳酸钙的体外模拟矿化实验,探讨两种可溶性有机质的浓度在控制CaCO_3结晶方面的作用.通过扫描电子显微镜及显微共聚焦拉曼光谱等方法对体外模拟矿化结果进行了观察和分析.结果表明,酸可溶蛋白对碳酸钙晶体的形貌和尺寸具有明显的调控作用,而对晶型无影响,不同浓度的酸可溶蛋白对碳酸钙晶体形貌影响差别不大,对尺寸的影响略有不同;水可溶蛋白对碳酸钙晶体的晶型、形貌及尺寸均有显著影响.不同浓度的水可溶蛋白对碳酸钙晶体的形貌和尺寸影响较大,对晶型有一定的调控作用.
이절강제기담수문석진주위실험원료,제취문석진주중적량충기질단백--산가용단백(ASM)화수가용유단백(WSM),병분별장기제비성일계렬불동농도적기질단백용액.이용상술량충불동농도적가용성유궤기질용액채용확산법개전료탄산개적체외모의광화실험,탐토량충가용성유궤질적농도재공제CaCO_3결정방면적작용.통과소묘전자현미경급현미공취초랍만광보등방법대체외모의광화결과진행료관찰화분석.결과표명,산가용단백대탄산개정체적형모화척촌구유명현적조공작용,이대정형무영향,불동농도적산가용단백대탄산개정체형모영향차별불대,대척촌적영향략유불동;수가용단백대탄산개정체적정형、형모급척촌균유현저영향.불동농도적수가용단백대탄산개정체적형모화척촌영향교대,대정형유일정적조공작용.
Freshwater pearls (aragonite pearls) were selected as the main experimental materials to study the biomineralization of calcium carbonate. Two kinds of soluble matrix proteins, i. e., acid soluble protein (ASM) and water soluble protein (WSM), were extracted from aragonite pearls to prepare a series of aqueous solutions with different concentrations. In vitro study was carried out by using the above-mentioned aqueous solutions with different concentrations in the diffusion method. The effects of concentrations of ASM and WSM aqueous solutions were discussed in the controlled crystallization of calcium carbonate. Scanning electron microscopy (SEM) and Raman spectroscopy were used to observe and analyze the results of in vitro biomineralization of calcium carbonate. The results show some differences in regulation and controlled crystallization of CaCO_3 between ASM and WSM aqueous solutions. The concentration of ASM aqueous solutions seems to play an important role in controlling the crystal morphology and size of calcium carbonate but it has no effect on polymorphism, while the polymorph, morphology and size of crystals are obviously influenced by the concentration of WSM aqueous solutions.