临床耳鼻咽喉头颈外科杂志
臨床耳鼻嚥喉頭頸外科雜誌
림상이비인후두경외과잡지
JOURNAL OF CLINICAL OTORHINOLARYNGOLOGY HEAD AND NECK SURGERY
2010年
4期
152-155
,共4页
刘杰%潘松%周远红%喻志华%刘立思
劉傑%潘鬆%週遠紅%喻誌華%劉立思
류걸%반송%주원홍%유지화%류립사
胰岛素样生长因子-Ⅰ受体%蛋白激酶C%喉肿瘤%癌,鳞状细胞
胰島素樣生長因子-Ⅰ受體%蛋白激酶C%喉腫瘤%癌,鱗狀細胞
이도소양생장인자-Ⅰ수체%단백격매C%후종류%암,린상세포
insulin-like growth factor-I receptor%protein kinase C%laryngeal neoplasms%carcinoma,squamous cell
目的:探讨胰岛素样生长因子-I受体(IGF-IR)和蛋白激酶C(PKC)在喉鳞状细胞癌中的表达情况及临床意义.方法:采用免疫组织化学SP法及Western blot技术检测60例喉鳞状细胞癌组织、25例癌旁组织及10例正常喉部组织中IGF-IR及PKC的表达情况.结果:IGF-IR及PKC在喉鳞状细胞癌中的表达阳性率较正常喉部组织及癌旁组织明显增高(P<0.05).IGF-IR在TNM分期Ⅲ～Ⅳ期、颈淋巴结转移及高分化癌患者中的表达水平分别较Ⅰ～Ⅱ期、无淋巴结转移及中低分化癌患者表达增强(P<0.05).PKC的阳性表达与喉癌临床分期及淋巴结转移相关(P<0.05).喉鳞状细胞癌组织中IGF-IR与PKC蛋白的表达呈正相关(rs=0.338,P<0.05).结论:IGF-IR及PKC在喉鳞状细胞癌中表达增高且两者密切相关,可能参与了喉癌的发生、发展过程,检测IGF-IR和PKC的表达对喉癌的早期诊断及治疗具有重要指导意义.
目的:探討胰島素樣生長因子-I受體(IGF-IR)和蛋白激酶C(PKC)在喉鱗狀細胞癌中的錶達情況及臨床意義.方法:採用免疫組織化學SP法及Western blot技術檢測60例喉鱗狀細胞癌組織、25例癌徬組織及10例正常喉部組織中IGF-IR及PKC的錶達情況.結果:IGF-IR及PKC在喉鱗狀細胞癌中的錶達暘性率較正常喉部組織及癌徬組織明顯增高(P<0.05).IGF-IR在TNM分期Ⅲ～Ⅳ期、頸淋巴結轉移及高分化癌患者中的錶達水平分彆較Ⅰ～Ⅱ期、無淋巴結轉移及中低分化癌患者錶達增彊(P<0.05).PKC的暘性錶達與喉癌臨床分期及淋巴結轉移相關(P<0.05).喉鱗狀細胞癌組織中IGF-IR與PKC蛋白的錶達呈正相關(rs=0.338,P<0.05).結論:IGF-IR及PKC在喉鱗狀細胞癌中錶達增高且兩者密切相關,可能參與瞭喉癌的髮生、髮展過程,檢測IGF-IR和PKC的錶達對喉癌的早期診斷及治療具有重要指導意義.
목적:탐토이도소양생장인자-I수체(IGF-IR)화단백격매C(PKC)재후린상세포암중적표체정황급림상의의.방법:채용면역조직화학SP법급Western blot기술검측60례후린상세포암조직、25례암방조직급10례정상후부조직중IGF-IR급PKC적표체정황.결과:IGF-IR급PKC재후린상세포암중적표체양성솔교정상후부조직급암방조직명현증고(P<0.05).IGF-IR재TNM분기Ⅲ～Ⅳ기、경림파결전이급고분화암환자중적표체수평분별교Ⅰ～Ⅱ기、무림파결전이급중저분화암환자표체증강(P<0.05).PKC적양성표체여후암림상분기급림파결전이상관(P<0.05).후린상세포암조직중IGF-IR여PKC단백적표체정정상관(rs=0.338,P<0.05).결론:IGF-IR급PKC재후린상세포암중표체증고차량자밀절상관,가능삼여료후암적발생、발전과정,검측IGF-IR화PKC적표체대후암적조기진단급치료구유중요지도의의.
Objective:To investigate the expression of IGF-IR and PKC and their clinical significance in laryngeal squamous cell carcinoma(LSCC).Method:The expression of IGF-IR and PKC protein were detected by immunohistochemistry and Western blot in 60 cases of LSCC, 25 cases of adjacent non-tumorous laryngeal epithelium and 10 cases of normal laryngeal epithelium.Result:The positive rate of IGF-IR and PKC expression in LSCC were significantly higher than that in normal laryngeal epithelium and adjacent non-tumorous laryngeal epithelium(P<0.05). IGF-IR positive expression were increased more in cases with stage Ⅲ-Ⅳ, cervical lymph node metastasis and well differentiation than that in cases with stageⅠ-Ⅱ, non-lymph node metastasis and poor/mediate differentiation, respectively(P<0.05).The positive rate of PKC in LSCC were correlated with clinical stage and cervical lymph node metastasis.There was a correlation between the expression of IGF-IR and PKC in LSCC.Conclusion:The overexpression of IGF-IR and PKC in the laryngeal carcinoma may play an important role in the carcinogenesis and development of LSCC. It is suggested that detecting the expression of IGF-IR and PKC can be used for early diagnosis and treatment of LSCC.
