中国全科医学
中國全科醫學
중국전과의학
CHINESE GENERAL PRACTICE
2010年
11期
1185-1188
,共4页
王江东%房辉%闫全志%周东浩%姚会君
王江東%房輝%閆全誌%週東浩%姚會君
왕강동%방휘%염전지%주동호%요회군
糖尿病,2型%白介素10%多态性,单核苷酸
糖尿病,2型%白介素10%多態性,單覈苷痠
당뇨병,2형%백개소10%다태성,단핵감산
Diabetes mellitus,type 2%Interleukin-10%Polymorphism,single nucleotide
目的 观察2型糖尿病患者血清IL-10水平及IL-10基因启动子592位点多态性与2型糖尿病易感性的关系.方法 选择224例2型糖尿病患者(DM组)和275例健康对照者(NC组).使用酶联免疫吸附法(ELISA)测定两组受检者血清IL-10水平;并从人体抗凝血白细胞中提取DNA,采用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)法分析IL-10-592基因多态性.结果 DM组血清IL-10水平为(21.1±5.2)pg/ml,对照组为(42.4±6.3)pg/ml,二者间差异有统计学意义(P＜0.01).DM组IL-10-592基因型频率分别为:纯合突变型(AA)36例(16.1%),杂合子(AC)122例(54.5%),野生型(CC)66例(29.4%);NC组:纯合突变型24例(8.7%),杂合子138例(50.2%),野生型113例(41.1%).DM组和NC组等位基因频率:A分别为43.3%和33.8%,C分别为56.7%和66.2%.两组IL-10-592基因型频率及等位基因频率间差异均有统计学意义(P＜0.01).结论 2型糖尿病患者血清IL-10水平显著低于健康者,IL-10可能对糖尿病发病及预后、保健、康复具有保护作用;IL-10基因启动子区592位点多态性可能与2型糖尿病的遗传易感性相关.
目的 觀察2型糖尿病患者血清IL-10水平及IL-10基因啟動子592位點多態性與2型糖尿病易感性的關繫.方法 選擇224例2型糖尿病患者(DM組)和275例健康對照者(NC組).使用酶聯免疫吸附法(ELISA)測定兩組受檢者血清IL-10水平;併從人體抗凝血白細胞中提取DNA,採用聚閤酶鏈反應-限製性片段長度多態性分析(PCR-RFLP)法分析IL-10-592基因多態性.結果 DM組血清IL-10水平為(21.1±5.2)pg/ml,對照組為(42.4±6.3)pg/ml,二者間差異有統計學意義(P＜0.01).DM組IL-10-592基因型頻率分彆為:純閤突變型(AA)36例(16.1%),雜閤子(AC)122例(54.5%),野生型(CC)66例(29.4%);NC組:純閤突變型24例(8.7%),雜閤子138例(50.2%),野生型113例(41.1%).DM組和NC組等位基因頻率:A分彆為43.3%和33.8%,C分彆為56.7%和66.2%.兩組IL-10-592基因型頻率及等位基因頻率間差異均有統計學意義(P＜0.01).結論 2型糖尿病患者血清IL-10水平顯著低于健康者,IL-10可能對糖尿病髮病及預後、保健、康複具有保護作用;IL-10基因啟動子區592位點多態性可能與2型糖尿病的遺傳易感性相關.
목적 관찰2형당뇨병환자혈청IL-10수평급IL-10기인계동자592위점다태성여2형당뇨병역감성적관계.방법 선택224례2형당뇨병환자(DM조)화275례건강대조자(NC조).사용매련면역흡부법(ELISA)측정량조수검자혈청IL-10수평;병종인체항응혈백세포중제취DNA,채용취합매련반응-한제성편단장도다태성분석(PCR-RFLP)법분석IL-10-592기인다태성.결과 DM조혈청IL-10수평위(21.1±5.2)pg/ml,대조조위(42.4±6.3)pg/ml,이자간차이유통계학의의(P＜0.01).DM조IL-10-592기인형빈솔분별위:순합돌변형(AA)36례(16.1%),잡합자(AC)122례(54.5%),야생형(CC)66례(29.4%);NC조:순합돌변형24례(8.7%),잡합자138례(50.2%),야생형113례(41.1%).DM조화NC조등위기인빈솔:A분별위43.3%화33.8%,C분별위56.7%화66.2%.량조IL-10-592기인형빈솔급등위기인빈솔간차이균유통계학의의(P＜0.01).결론 2형당뇨병환자혈청IL-10수평현저저우건강자,IL-10가능대당뇨병발병급예후、보건、강복구유보호작용;IL-10기인계동자구592위점다태성가능여2형당뇨병적유전역감성상관.
Objective To investigate the relationship of serum IL-10 level and its gene promoter 592 polymorphism to type 2 diabetes mellitus(T2DM) susceptibility.Methods Two hundred twenty-four T2DM patients (DM group) and 275 normal subjects (control group) were enrolled from Tangshan region of Hebei Province.DNA was extracted from white cells in human anticoagulant plasma to analyze IL-10-592 gene polymorphism by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).Results There was significant difference in serum IL-10 level between groups DM (21.1±5.2 pg/ml) and control (42.4±6.3 pg/ml) (P＜0.01).IL-10-592 genotypic frequencies were 16.1%(AA),54.5%(AC),29.4%(CC),respectively,in DM group,and 8.7%(AA),50.2%(AC),41.1%(CC),respectively,in control group.The allele frequencies of groups DM and NC were 43.3%,33.8%,respectively,for A,and 56.7%,66.2%,respectively for C.The difference was significant between 2 groups (P＜0.01).Conclusion The IL-10 levels are significantly lower in T2DM patients than in healthy subjects.IL-10 may play a protective role in diabetic incidence,health care and rehabilitation.IL-10-592 polymorphism may be related to T2DM genetic susceptibility.
