中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2011年
7期
621-623
,共3页
条件恐惧性刺激%新生神经细胞%海马%大鼠
條件恐懼性刺激%新生神經細胞%海馬%大鼠
조건공구성자격%신생신경세포%해마%대서
Conditioned fear stimulus%Neurogenesis%Hippocampus%Rat
目的 研究条件恐惧刺激后大鼠海马内新生神经细胞的表达变化,探讨恐惧情绪性记忆的神经牛物学机制.方法 24只成年雄性大鼠随机分成条件恐惧刺激组和对照组,实验动物刺激前3 d腹腔内注射Brdu标记神经干细胞,实验组动物采用单调声配对电击大鼠足底造成条件恐惧刺激模型,对照组仅给予单调声刺激.刺激前和刺激后7,14d分别行动物行为学检测(静止不动时间);动物存活7,14d后处死,冰冻切片行Brdu的免疫组织化学染色.结果 条件恐惧刺激后,行为学检测结果显示实验组大鼠静止不动时间百分比显著高于对照组[分别为(88.68%、91.33%)和(11.18%、14.23%),P<0.01]及电击前(24.11%、16.11%,P<0.01);Brdu免疫组化染色显示条件恐惧刺激后实验组7,14d组大鼠海马齿状回内Brdu免疫阳性细胞少于同时间点的对照组[分别为(1364±21)个、(296±22)个和(1567±28)个、(580±5)个,14d组差异具有显著性P<0.05].结论 条件恐惧刺激后大鼠海马齿状回新生神经元的减少可能与恐惧等情绪性记忆有关.
目的 研究條件恐懼刺激後大鼠海馬內新生神經細胞的錶達變化,探討恐懼情緒性記憶的神經牛物學機製.方法 24隻成年雄性大鼠隨機分成條件恐懼刺激組和對照組,實驗動物刺激前3 d腹腔內註射Brdu標記神經榦細胞,實驗組動物採用單調聲配對電擊大鼠足底造成條件恐懼刺激模型,對照組僅給予單調聲刺激.刺激前和刺激後7,14d分彆行動物行為學檢測(靜止不動時間);動物存活7,14d後處死,冰凍切片行Brdu的免疫組織化學染色.結果 條件恐懼刺激後,行為學檢測結果顯示實驗組大鼠靜止不動時間百分比顯著高于對照組[分彆為(88.68%、91.33%)和(11.18%、14.23%),P<0.01]及電擊前(24.11%、16.11%,P<0.01);Brdu免疫組化染色顯示條件恐懼刺激後實驗組7,14d組大鼠海馬齒狀迴內Brdu免疫暘性細胞少于同時間點的對照組[分彆為(1364±21)箇、(296±22)箇和(1567±28)箇、(580±5)箇,14d組差異具有顯著性P<0.05].結論 條件恐懼刺激後大鼠海馬齒狀迴新生神經元的減少可能與恐懼等情緒性記憶有關.
목적 연구조건공구자격후대서해마내신생신경세포적표체변화,탐토공구정서성기억적신경우물학궤제.방법 24지성년웅성대서수궤분성조건공구자격조화대조조,실험동물자격전3 d복강내주사Brdu표기신경간세포,실험조동물채용단조성배대전격대서족저조성조건공구자격모형,대조조부급여단조성자격.자격전화자격후7,14d분별행동물행위학검측(정지불동시간);동물존활7,14d후처사,빙동절편행Brdu적면역조직화학염색.결과 조건공구자격후,행위학검측결과현시실험조대서정지불동시간백분비현저고우대조조[분별위(88.68%、91.33%)화(11.18%、14.23%),P<0.01]급전격전(24.11%、16.11%,P<0.01);Brdu면역조화염색현시조건공구자격후실험조7,14d조대서해마치상회내Brdu면역양성세포소우동시간점적대조조[분별위(1364±21)개、(296±22)개화(1567±28)개、(580±5)개,14d조차이구유현저성P<0.05].결론 조건공구자격후대서해마치상회신생신경원적감소가능여공구등정서성기억유관.
Objective To investigate neurogenesis in the dentate gyrus of adult rat following conditioned fear stimulus.Methods 24 adult male rats were randomly divided into conditioned fear stimulus group and control group,and all rats were intraperitoneally injected bromodeoxyuridine(BrdU)3 days before conditioned fear stimuli.Animals in conditioned fear stimulus group were given monotone matching foot shock and made conditioned fear stimulus model,animals in control group only give monotone.Freezing time was detected before stimuli and 7th,14th days after stimuli,then rats were sacrificed 7th or 14th day after fear conditioned stimulus,and immunohistochemistry for Brdu was detected.Results Behavioral assessment showed that the percents of freezing time in conditioned fear stimulus group 7th,14th days after conditioned fear stimulus were significantly high(88.68%,91.33%)than those in control group(11.18%,14.23%,P<0.01)and before stimulus(24.11%,16.11%,P<0.01).Compared to control group(1567±28,580±5),Brdu positive cells in dentate gyrus of rat in conditioned fear stimulus group(1364±21,296±22)were decreased,and it reached to a statistical significance on 14th day (P<0.05).Conclusion The decreased neurogenesis in the dentate gyrus of adult rat following conditioned fear stimuli may have relation to fear and other emotion memory.
