中华耳鼻咽喉头颈外科杂志
中華耳鼻嚥喉頭頸外科雜誌
중화이비인후두경외과잡지
CHINESE JOURNAL OF OTORHINOLARYNGOLOGY HEAD AND NECK SURGERY
2008年
2期
134-138
,共5页
李钦%张永东%孙崇伟%陈彦林%杜英华%赵广娟%张大良
李欽%張永東%孫崇偉%陳彥林%杜英華%趙廣娟%張大良
리흠%장영동%손숭위%진언림%두영화%조엄연%장대량
模型,动物%鼻炎,变应性,常年性%干扰素Ⅱ型%GATA3转录因子
模型,動物%鼻炎,變應性,常年性%榦擾素Ⅱ型%GATA3轉錄因子
모형,동물%비염,변응성,상년성%간우소Ⅱ형%GATA3전록인자
Models,animal%Rhinitis,allergic,perennial%Interferon typeⅡ%GATA3 transcription factor
目的 探讨鼻腔吸入γ干扰素(interferon gamma,IFN-γ)对大鼠变应性鼻炎(allergic rhinitis,AR)的治疗作用及可能机制.方法 采用卵白蛋白、氢氧化铝建立大鼠AR模型,分为阳性对照组(B组)、IFN-γ组(c组)和丙酸倍氯米松组(D组),每组8只大鼠,分别于模型建立后第31～38天,每只每日每侧鼻腔滴入磷酸盐缓冲液50μl、IFN-γ1 μg和丙酸倍氯米松3.5 μg,另设阴性对照组(A组)大鼠8只.第39天取鼻腔灌洗液测定细胞成分、白细胞介素4和白细胞介素5浓度;取血测定血浆IgE水平;黏膜切片观察鼻腔组织病理学改变及GATA-3的表达.结果 C组鼻腔灌洗液中的嗜酸粒细胞数量(-x±s,下同)为(O.005±0.003)×104/ml,明显低于B组(0.225±0.060)x104/ml(P<0.01);C组鼻腔灌洗液中的白细胞介素4为(7.8±3.5)pg/ml,白细胞介素5为(12.5±4.3)pg/ml,均低于B组(P值均<0.01);C组血浆中总IgE为(38.5±9.6)μg/ml,卵白蛋白特异性IgE为(19.8±5.4)IU/ml,均低于B组(P值均<0.01).B组大鼠鼻腔黏膜充血、水肿,黏膜层增厚,并有嗜酸细胞为主的炎性细胞浸润,而c组大鼠上述炎性症状改变减轻.免疫组化显示B组鼻腔组织中GATA-3表达增加,而C组的表达减少.结论 鼻腔吸入IFN-γ可以抑制AR大鼠白细胞介素4和白细胞介素5的合成,抑制嗜酸粒细胞在鼻腔内的炎性浸润,降低血浆中总IgE和卵白蛋白特异性IgE水平,其机制可能与阻断GATA-3表达,从而继发抑制Th2型反应有关.
目的 探討鼻腔吸入γ榦擾素(interferon gamma,IFN-γ)對大鼠變應性鼻炎(allergic rhinitis,AR)的治療作用及可能機製.方法 採用卵白蛋白、氫氧化鋁建立大鼠AR模型,分為暘性對照組(B組)、IFN-γ組(c組)和丙痠倍氯米鬆組(D組),每組8隻大鼠,分彆于模型建立後第31～38天,每隻每日每側鼻腔滴入燐痠鹽緩遲液50μl、IFN-γ1 μg和丙痠倍氯米鬆3.5 μg,另設陰性對照組(A組)大鼠8隻.第39天取鼻腔灌洗液測定細胞成分、白細胞介素4和白細胞介素5濃度;取血測定血漿IgE水平;黏膜切片觀察鼻腔組織病理學改變及GATA-3的錶達.結果 C組鼻腔灌洗液中的嗜痠粒細胞數量(-x±s,下同)為(O.005±0.003)×104/ml,明顯低于B組(0.225±0.060)x104/ml(P<0.01);C組鼻腔灌洗液中的白細胞介素4為(7.8±3.5)pg/ml,白細胞介素5為(12.5±4.3)pg/ml,均低于B組(P值均<0.01);C組血漿中總IgE為(38.5±9.6)μg/ml,卵白蛋白特異性IgE為(19.8±5.4)IU/ml,均低于B組(P值均<0.01).B組大鼠鼻腔黏膜充血、水腫,黏膜層增厚,併有嗜痠細胞為主的炎性細胞浸潤,而c組大鼠上述炎性癥狀改變減輕.免疫組化顯示B組鼻腔組織中GATA-3錶達增加,而C組的錶達減少.結論 鼻腔吸入IFN-γ可以抑製AR大鼠白細胞介素4和白細胞介素5的閤成,抑製嗜痠粒細胞在鼻腔內的炎性浸潤,降低血漿中總IgE和卵白蛋白特異性IgE水平,其機製可能與阻斷GATA-3錶達,從而繼髮抑製Th2型反應有關.
목적 탐토비강흡입γ간우소(interferon gamma,IFN-γ)대대서변응성비염(allergic rhinitis,AR)적치료작용급가능궤제.방법 채용란백단백、경양화려건립대서AR모형,분위양성대조조(B조)、IFN-γ조(c조)화병산배록미송조(D조),매조8지대서,분별우모형건립후제31～38천,매지매일매측비강적입린산염완충액50μl、IFN-γ1 μg화병산배록미송3.5 μg,령설음성대조조(A조)대서8지.제39천취비강관세액측정세포성분、백세포개소4화백세포개소5농도;취혈측정혈장IgE수평;점막절편관찰비강조직병이학개변급GATA-3적표체.결과 C조비강관세액중적기산립세포수량(-x±s,하동)위(O.005±0.003)×104/ml,명현저우B조(0.225±0.060)x104/ml(P<0.01);C조비강관세액중적백세포개소4위(7.8±3.5)pg/ml,백세포개소5위(12.5±4.3)pg/ml,균저우B조(P치균<0.01);C조혈장중총IgE위(38.5±9.6)μg/ml,란백단백특이성IgE위(19.8±5.4)IU/ml,균저우B조(P치균<0.01).B조대서비강점막충혈、수종,점막층증후,병유기산세포위주적염성세포침윤,이c조대서상술염성증상개변감경.면역조화현시B조비강조직중GATA-3표체증가,이C조적표체감소.결론 비강흡입IFN-γ가이억제AR대서백세포개소4화백세포개소5적합성,억제기산립세포재비강내적염성침윤,강저혈장중총IgE화란백단백특이성IgE수평,기궤제가능여조단GATA-3표체,종이계발억제Th2형반응유관.
Objective To investigate the effects and mechanism of intranasal interferon gamma (IFN-γ)in the treatment of allergic rhinifis.Methods Ovalbumin(OVA)absorbed to aluminum hydroxide was used to construct the allergic rhinitis model(group C),and the normal control group(group A),the allergic rhinitis model group(group B)and beclomethasone dipropionate group(group D) consisted of 8 rats for each.PBS 50 μl was absorbed to group B,IFN-γ1 μg was absorbed to group C and beclomethasone dipropionate 3.5 μg was absorbed to group D on day 31 to day 38 once daily once nasal cavity.The nasal lavage fluid was collected on day 39,and the cellular constituents,levels of interleukin-4 (IL-4),interleukin-5(IL-5)and IgE were determined,together with the pathologic changes and expression of GATA-3 were observed.Results Decrease of eosinophils[(0.0050.003)×104/ml,-x±s]was seen in nasal lavage fluid of group C as comparing with group B[(0.225±0.060)×104/ml,(P<0.01)],and the levels of IL-4(7.8±3.5)pg/ml and IL-5(12.5±4.3)pg/ml decreased significantly in comparing with group B(P<0.01).The serum levels of total IgE(38.5±9.6) g/ml and ovalbumin-specific IgE(19.8±5.4)IU/ml decreased significantly in comparing with those of group B(P<0.01).In group B,mucosal congestion and edema thickening with inflammatory cells infiltration mainly of eosinophils;in group C,the above mentioned changes were much more ameliorated.Immunohistochemistry showed significant increase of GATA-3 expression in the nosal tissue of group B but much lesser than that in group C.Conclusions IFN-γcan inhibit the composition of IL-4 and IL-5.and inhibit the airway inflammation with eosinophilic infiltration and the serum levels of total IgE and ovalbumin specific IgE,probably through the mechanism of restraining the Th2 reaction by blockade of GATA-3 expression in the nasal tissue.
