中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2012年
3期
311-313
,共3页
多塞平%注射,脊髓%药物毒性
多塞平%註射,脊髓%藥物毒性
다새평%주사,척수%약물독성
Doxepin%Injections,spinal%Drug toxicity
目的 评价鞘内注射不同浓度多塞平对大鼠的神经毒性.方法 鞘内置管成功的成年雄性Wistar大鼠24只,体重250～300 g,采用随机数字表法,将大鼠随机分为4组(n=6).D10组、D20组和D40组分别鞘内注射10、20和40 mmol/L多塞平0.2 μl/g,N组鞘内注射等容量生理盐水.于鞘内注射后6h时取大鼠腰膨大脊髓背角和脊神经后根,透射电镜下观察超微结构；采用ELISA法测定脊髓髓鞘碱性蛋白(MBP)含量.结果 N组大鼠脊髓背角、脊神经后根超微结构正常；随多塞平浓度增加,脊髓神经元染色质边集、核膜局部凹陷,胶质细胞核固缩,线粒体空泡样变性等细胞凋亡与坏死改变；脊神经后根神经纤维出现线粒体髓样变及空泡样改变.脊髓MBP含量随多塞平浓度增加而降低(P＜0.05).结论 大鼠鞘内注射多塞平可产生脊髓和外周神经毒性,且呈浓度依赖性.
目的 評價鞘內註射不同濃度多塞平對大鼠的神經毒性.方法 鞘內置管成功的成年雄性Wistar大鼠24隻,體重250～300 g,採用隨機數字錶法,將大鼠隨機分為4組(n=6).D10組、D20組和D40組分彆鞘內註射10、20和40 mmol/L多塞平0.2 μl/g,N組鞘內註射等容量生理鹽水.于鞘內註射後6h時取大鼠腰膨大脊髓揹角和脊神經後根,透射電鏡下觀察超微結構；採用ELISA法測定脊髓髓鞘堿性蛋白(MBP)含量.結果 N組大鼠脊髓揹角、脊神經後根超微結構正常；隨多塞平濃度增加,脊髓神經元染色質邊集、覈膜跼部凹陷,膠質細胞覈固縮,線粒體空泡樣變性等細胞凋亡與壞死改變；脊神經後根神經纖維齣現線粒體髓樣變及空泡樣改變.脊髓MBP含量隨多塞平濃度增加而降低(P＜0.05).結論 大鼠鞘內註射多塞平可產生脊髓和外週神經毒性,且呈濃度依賴性.
목적 평개초내주사불동농도다새평대대서적신경독성.방법 초내치관성공적성년웅성Wistar대서24지,체중250～300 g,채용수궤수자표법,장대서수궤분위4조(n=6).D10조、D20조화D40조분별초내주사10、20화40 mmol/L다새평0.2 μl/g,N조초내주사등용량생리염수.우초내주사후6h시취대서요팽대척수배각화척신경후근,투사전경하관찰초미결구；채용ELISA법측정척수수초감성단백(MBP)함량.결과 N조대서척수배각、척신경후근초미결구정상；수다새평농도증가,척수신경원염색질변집、핵막국부요함,효질세포핵고축,선립체공포양변성등세포조망여배사개변；척신경후근신경섬유출현선립체수양변급공포양개변.척수MBP함량수다새평농도증가이강저(P＜0.05).결론 대서초내주사다새평가산생척수화외주신경독성,차정농도의뢰성.
Objective To investigate the neurotoxicity of intrathecal (IT) doxepin in rats.Methods Twenty-four adult male Wistar rats weighing 250-300 g in which intrathecal micro-catheter was successfully implanted without complications were randomly divided into 4 groups ( n =6 each):control group ( group N ) and 3 doxepin groups receiving IT doxepin 40,20 and 10 mmol/L 0.2 μl/g respectively (groups D40,D20,D10 ).In N group normal saline was injected IT instead of doxepin.The animals were killed at 6 h after IT administration.The lumbar segment of the spinal cord was removed for microscopic examination and determination of myelin basic protein (MBP) content in the spinal cord using a double-antibody sandwich (ELISA).Results The severity of neuronal damage and decrease in MBP content in the spinal cord induced by IT doxepin were dose-dependent.Conclusion The neurotoxicity induced by IT doxepin is dose-dependent.
