CAJ | 학술논문

目的探索超声辐射影响过氧化氢酶(CAT)的催化效果及其作用机理.方法本试验用不同参数的超声波处理酵母CAT,测定处理前后酶活性变化、催化动力学的变化以及光谱学的变化.结果试验结果表明,在30℃的条件下,用16 kHz、50 W的超声波处理酵母CAT 2 min,酶活力可提高126.35%.经超声波处理后的CAT分子荧光强度有所增加.超声波处理后,CAT的紫外吸收光谱基本不改变,说明超声作用并没有改变CAT的分子构型.而超声波处理后,CAT的紫外差示光谱分别出现正峰和负峰,说明超声波使CAT的分子构象发生了改变.对超声波处理前后的CAT分别进行动力学分析,结果表明超声波处理使酶的米氏常数(Km)和最大反应速度(Vmax)都增大.结论从试验结果得出结论,适宜参数超声波处理酵母CAT后酶活性升高,可能是超声波改变酶分子的构象所引起.
목적 탐색초성복사영향과양화경매(CAT)적최화효과급기작용궤리.방법 본시험용불동삼수적초성파처리효모CAT,측정처리전후매활성변화、최화동역학적변화이급광보학적변화.결과 시험결과표명,재30℃적조건하,용16 kHz、50 W적초성파처리효모CAT 2 min,매활력가제고126.35%.경초성파처리후적CAT분자형광강도유소증가.초성파처리후,CAT적자외흡수광보기본불개변,설명초성작용병몰유개변CAT적분자구형.이초성파처리후,CAT적자외차시광보분별출현정봉화부봉,설명초성파사CAT적분자구상발생료개변.대초성파처리전후적CAT분별진행동역학분석,결과표명초성파처리사매적미씨상수(Km)화최대반응속도(Vmax)도증대.결론 종시험결과득출결론,괄의삼수초성파처리효모CAT후매활성승고,가능시초성파개변매분자적구상소인기.
Objective To study the effect of ultrasound on the activity of catalase(CAT) and its action mechanism.Methods Ultrasound with different parameters was used to treat yeast CAT.Changes of enzyme activity,kinetics,and spectra were detected before and after treatment.Results It indicated that the activity of catalase from yeast (Saccharomyces cerevisiae) could be increased by 126.35% via treatment with ultrasound of 16 kHz and 50 W at 30℃ for 2 min.CAT treated by ultrasound presented higher fluorescence emission intensity.The ultra violet(UV) absorption spectrum had no obvious change after ultrasound treatment.This indicated that the ultrasound treatment did not change the molecular configuration of CAT.But after the treatment,UV differential spectrum showed positive and negative peaks.This indicated that the molecular conformation changed obviously.The results of enzyme kinetics analysis indicated that both Km and Vmax increased after ultrasound treatment.Conclusion Ultrasound with appropriate parameters cna increase the activity of CAT.This misht be caused by the change of conformation of the enzyme molecules caused by ultrasound.