中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2010年
22期
1522-1525
,共4页
江虹%曾婷婷%黄骥斌%毛志刚%曾素根
江虹%曾婷婷%黃驥斌%毛誌剛%曾素根
강홍%증정정%황기빈%모지강%증소근
血细胞计数%评价研究%诊断设备%自动分析
血細胞計數%評價研究%診斷設備%自動分析
혈세포계수%평개연구%진단설비%자동분석
Blood cell count%Evaluation studies%Diagnositic equipment%Autoanalysis
目的 对血细胞分析复检规则进行临床应用的分析评价.方法 应用Sysmex XE-2100血细胞分析仪检测四川大学华西医院8820例患者的静脉血标本,并同时进行双盲法人工显微镜检查(镜检).根据中华医学会检验医学分会XE-2100血细胞分析仪复检标准制定协作组制定的Sysmex XE-2100自动血细胞分析和白细胞分类复检规则进行统计分析,计算检测的真假阳性率、真假阴性率、灵敏性、特异性、阳性预测值、阴性预测值、总有效率、检验效能和复检率,同时分析四川大学华西医院实验医学科根据实际情况进行适当调整后的复检规则的临床效能.结果 8820 份标本检测的真阳性率为13.0%(1149 份),假阳性率27.2%(2397份),真阴性率55.3%(4876份),假阴性率4.5%(398份),灵敏性74.3%,特异性67.0%,阳性预测值32.4%,阴性预测值92.5%,总有效率68.3%,检验效能21.2%,复检率40.2%.对复检规则进行调整后,复检率下降至33.8%,总有效率提高至74.7%.8820份标本中2069份需镜检人工白细胞分类,占23.5%,其中847份标本发现非正常细胞,未发现白血病细胞的漏检.结论 Sysmex XE-2100自动血细胞分析和白细胞分类复检规则对临床工作有很大帮助.各实验室在制定血常规检测复检规则时应根据患者来源、仪器性能等对复检规则进行一定的调整,以更好地满足临床应用.
目的 對血細胞分析複檢規則進行臨床應用的分析評價.方法 應用Sysmex XE-2100血細胞分析儀檢測四川大學華西醫院8820例患者的靜脈血標本,併同時進行雙盲法人工顯微鏡檢查(鏡檢).根據中華醫學會檢驗醫學分會XE-2100血細胞分析儀複檢標準製定協作組製定的Sysmex XE-2100自動血細胞分析和白細胞分類複檢規則進行統計分析,計算檢測的真假暘性率、真假陰性率、靈敏性、特異性、暘性預測值、陰性預測值、總有效率、檢驗效能和複檢率,同時分析四川大學華西醫院實驗醫學科根據實際情況進行適噹調整後的複檢規則的臨床效能.結果 8820 份標本檢測的真暘性率為13.0%(1149 份),假暘性率27.2%(2397份),真陰性率55.3%(4876份),假陰性率4.5%(398份),靈敏性74.3%,特異性67.0%,暘性預測值32.4%,陰性預測值92.5%,總有效率68.3%,檢驗效能21.2%,複檢率40.2%.對複檢規則進行調整後,複檢率下降至33.8%,總有效率提高至74.7%.8820份標本中2069份需鏡檢人工白細胞分類,佔23.5%,其中847份標本髮現非正常細胞,未髮現白血病細胞的漏檢.結論 Sysmex XE-2100自動血細胞分析和白細胞分類複檢規則對臨床工作有很大幫助.各實驗室在製定血常規檢測複檢規則時應根據患者來源、儀器性能等對複檢規則進行一定的調整,以更好地滿足臨床應用.
목적 대혈세포분석복검규칙진행림상응용적분석평개.방법 응용Sysmex XE-2100혈세포분석의검측사천대학화서의원8820례환자적정맥혈표본,병동시진행쌍맹법인공현미경검사(경검).근거중화의학회검험의학분회XE-2100혈세포분석의복검표준제정협작조제정적Sysmex XE-2100자동혈세포분석화백세포분류복검규칙진행통계분석,계산검측적진가양성솔、진가음성솔、령민성、특이성、양성예측치、음성예측치、총유효솔、검험효능화복검솔,동시분석사천대학화서의원실험의학과근거실제정황진행괄당조정후적복검규칙적림상효능.결과 8820 빈표본검측적진양성솔위13.0%(1149 빈),가양성솔27.2%(2397빈),진음성솔55.3%(4876빈),가음성솔4.5%(398빈),령민성74.3%,특이성67.0%,양성예측치32.4%,음성예측치92.5%,총유효솔68.3%,검험효능21.2%,복검솔40.2%.대복검규칙진행조정후,복검솔하강지33.8%,총유효솔제고지74.7%.8820빈표본중2069빈수경검인공백세포분류,점23.5%,기중847빈표본발현비정상세포,미발현백혈병세포적루검.결론 Sysmex XE-2100자동혈세포분석화백세포분류복검규칙대림상공작유흔대방조.각실험실재제정혈상규검측복검규칙시응근거환자래원、의기성능등대복검규칙진행일정적조정,이경호지만족림상응용.
Objective To evaluate the review criteria for complete blood analysis.Methods A total of 8820 blood samples taken at our hospital were detected both by the Sysmex XE-2100 automated blood cell analyzer and double-blind method microscopy.The performance of two review criteria were evaluated by statistical analysis.The parameters,such as true and false positive rates,true and false negative rates,sensitivity,specificity,positive predictive value,negative predictive value,total effective rate,test effectiveness and re-examination rate were calculated according to the criteria of XE-2100 hematology analyzer re-examination standards coordination group of Society of Laboratory Medicine of Chinese Medical Association.And a study was conducted to determine the improved clinical effectiveness over the criteria.Results The values of true and false positive rates,true and false negative rates,sensitivity,specificity,positive predictive values,negative predictive value,total effective rate,test effectiveness and reexamination rate were 13.0%,27.2%,55.3%,4.5%,74.3%,67.0%,32.4%,92.5%,68.3%,21.2%,and 40.2% respectively.The re-examination rate decreased to 33.8% and the total effective rate increased to 74.7% according to our improved review criteria.And there Was no missed diagnosis of leukemic cells.It was found that 2069 samples (23.5%) needed manual microscopy for WBC differential analysis,847 had abnormal cells and no leukemia was missed.Conclusions The automatic blood cell analyzer and WBC differential analysis review criteria have great clinical utilities.The review criteria of blood cell analysis may be improved according to patient source and instrument performance so as to better meet the needs of clinical application.
